Rat prostacycline,PGI2 ELISA Kit

產品詳情

文獻引用 (6) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

大鼠前列環素(PGI2)酶聯免疫試劑盒（CSB-E13706r）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的PGI2含量。PGI2，即前列環素，是一種花生四烯酸的代謝產物，具有廣泛的生物學功能，如血管舒張、抗血小板聚集和免疫調節等。其研究機制涉及與特定受體結合，激活G蛋白，進而調節細胞內信號傳導。近年來，PGI2在治療肺動脈高壓等疾病中的應用研究取得了顯著進展。試劑盒檢測范圍為0.25 ng/mL-50 ng/mL，適用于科研領域中對大鼠模型血管功能研究、抗血栓藥物作用機制探索、炎癥相關疾病動物實驗的分子標志物檢測，以及組織微環境中PGI2代謝動態分析，為心血管生物學、藥理學及病理機制研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

PGI2
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.25 ng/mL-50 ng/mL
靈敏度：

0.125 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Biochemicals
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat PGI2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	92-104
1:2	Average %	94
	Range %	91-98
1:4	Average %	102
	Range %	98-106
1:8	Average %	88
	Range %	82-94

回收率：

The recovery of rat PGI2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	103	98-107

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.