Mouse prostacycline,PGI2 ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (2) 說(shuō)明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

小鼠前列環(huán)素(PGI2)酶聯(lián)免疫試劑盒（CSB-E13698m）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的PGI2含量。PGI2即前列環(huán)素，是一種具有強(qiáng)大血管舒張和血小板聚集抑制作用的內(nèi)源性前列腺素。其合成主要由血管內(nèi)皮細(xì)胞完成。研究機(jī)制上，它通過(guò)與特定受體結(jié)合，激活腺苷酸環(huán)化酶，升高細(xì)胞內(nèi)cAMP水平，從而發(fā)揮生物學(xué)效應(yīng)。試劑盒檢測(cè)范圍為25 pg/mL-1000 pg/mL，該產(chǎn)品適用于心血管疾病模型、抗血栓藥物機(jī)制、內(nèi)皮功能評(píng)估等科研場(chǎng)景，尤其適合探究PGI2在炎癥微環(huán)境、血管舒縮調(diào)節(jié)及組織損傷修復(fù)中的分子作用，為相關(guān)基礎(chǔ)研究提供精準(zhǔn)的定量數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說(shuō)明書。
縮寫：

PGI2
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

25 pg/mL-1000 pg/mL
靈敏度：

25 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Biochemicals
測(cè)定原理：

quantitative
測(cè)定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse PGI2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	86-108
1:2	Average %	88
	Range %	82-100
1:4	Average %	95
	Range %	85-105
1:8	Average %	93
	Range %	84-100

回收率：

The recovery of mouse PGI2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	91-108
EDTA plasma (n=4)	96	87-105

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.