Human prostacycline,PGI2 ELISA Kit

產(chǎn)品詳情

文獻引用 (3) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人前列環(huán)素(PGI2)酶聯(lián)免疫試劑盒（CSB-E09591h）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的PGI2含量。PGI2即前列環(huán)素，是一種具有強大擴血管及抗血小板聚集作用的生物活性物質(zhì)。可激活腺苷酸環(huán)化酶，升高血小板內(nèi)cAMP水平，抑制血小板聚集并舒張血管，在心血管疾病等方面的防治研究上具有重要意義。試劑盒檢測范圍為50 pg/mL-2000 pg/mL，該產(chǎn)品適用于心血管機制研究、藥物對血管內(nèi)皮功能影響的體外評估，以及炎癥或血栓性疾病模型中PGI2動態(tài)變化的定量分析，為探索血管調(diào)節(jié)機制、抗血小板藥物開發(fā)等科研方向提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

PGI2
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma
檢測范圍：

50 pg/mL-2000 pg/mL
靈敏度：

50 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Biochemicals
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human PGI2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	86-101
1:2	Average %	94
	Range %	86-100
1:4	Average %	94
	Range %	91-96
1:8	Average %	95
	Range %	85-105

回收率：

The recovery of human PGI2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	88-98
EDTA plasma (n=4)	96	92-100

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.