(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Western Blot
Positive WB detected in: Raji whole cell lysate(30μg),Hela whole cell lysate(30μg)
All lanes: IMPDH 2 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 56 kDa
Observed band size: 56 kDa
Exposure time：2min

Overlay Peak curve showing jurkat cells stained with CSB-RA210986A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

Western blot
All lanes: IMPDH2 antibody at 12µg/ml
Lane 1: Mouse heart tissue
Lane 2: A549 whole cell lysate
Lane 3: Hela whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 56 kDa
Observed band size: 56 kDa

Immunohistochemistry of paraffin-embedded human lung cancer using CSB-PA011701LA01HU at dilution of 1:100

Immunofluorescent analysis of Hela cells using CSB-PA011701LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)