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重組蛋白

產品名稱 貨號 規格
Recombinant Clostridium thermocellum ATP-dependent helicase/nuclease subunit A (addA), partial CSB-YP386397DUT
CSB-EP386397DUT
CSB-BP386397DUT
CSB-MP386397DUT
CSB-EP386397DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Cysteine--tRNA ligase (cysS) CSB-YP386398DUT
CSB-EP386398DUT
CSB-BP386398DUT
CSB-MP386398DUT
CSB-EP386398DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Endoglucanase SS (celS), partial CSB-YP386399DUT
CSB-EP386399DUT
CSB-BP386399DUT
CSB-MP386399DUT
CSB-EP386399DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP386400DUT
CSB-EP386400DUT
CSB-BP386400DUT
CSB-MP386400DUT
CSB-EP386400DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Carbon storage regulator homolog (csrA) CSB-YP386401DUT
CSB-EP386401DUT
CSB-BP386401DUT
CSB-MP386401DUT
CSB-EP386401DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum S-adenosylmethionine synthase (metK) CSB-YP386402DUT
CSB-EP386402DUT
CSB-BP386402DUT
CSB-MP386402DUT
CSB-EP386402DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Undecaprenyl-diphosphatase (uppP), partial CSB-YP386403DUT
CSB-EP386403DUT
CSB-BP386403DUT
CSB-MP386403DUT
CSB-EP386403DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP386404DUT
CSB-EP386404DUT
CSB-BP386404DUT
CSB-MP386404DUT
CSB-EP386404DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum 50S ribosomal protein L34 (rpmH) CSB-YP386405DUT
CSB-EP386405DUT
CSB-BP386405DUT
CSB-MP386405DUT
CSB-EP386405DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP386406DUT
CSB-EP386406DUT
CSB-BP386406DUT
CSB-MP386406DUT
CSB-EP386406DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP386407DUT
CSB-EP386407DUT
CSB-BP386407DUT
CSB-MP386407DUT
CSB-EP386407DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase CSB-YP386408DUT
CSB-EP386408DUT
CSB-BP386408DUT
CSB-MP386408DUT
CSB-EP386408DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Putative septation protein spoVG (spoVG) CSB-YP386409DUT
CSB-EP386409DUT
CSB-BP386409DUT
CSB-MP386409DUT
CSB-EP386409DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Dihydroxy-acid dehydratase (ilvD), partial CSB-YP386410DUT
CSB-EP386410DUT
CSB-BP386410DUT
CSB-MP386410DUT
CSB-EP386410DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum 30S ribosomal protein S12 (rpsL) CSB-YP386411DUT
CSB-EP386411DUT
CSB-BP386411DUT
CSB-MP386411DUT
CSB-EP386411DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Trigger factor (tig) CSB-YP386412DUT
CSB-EP386412DUT
CSB-BP386412DUT
CSB-MP386412DUT
CSB-EP386412DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Endoglucanase C (celC) CSB-YP386413DUT
CSB-EP386413DUT
CSB-BP386413DUT
CSB-MP386413DUT
CSB-EP386413DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum ATP phosphoribosyltransferase regulatory subunit (hisZ) CSB-YP386414DUT
CSB-EP386414DUT
CSB-BP386414DUT
CSB-MP386414DUT
CSB-EP386414DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum ATP phosphoribosyltransferase (hisG) CSB-YP386415DUT
CSB-EP386415DUT
CSB-BP386415DUT
CSB-MP386415DUT
CSB-EP386415DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Transcription elongation factor GreA (greA) CSB-YP386416DUT
CSB-EP386416DUT
CSB-BP386416DUT
CSB-MP386416DUT
CSB-EP386416DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產生大量蛋白質、修改基因序列和制造有用的商業產品。重組蛋白的技術資源在重組蛋白的開發過程中,除了表達系統之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據他們開發重組蛋白的經驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產,在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經過多年的發展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.jhhuazhong.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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