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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Clostridium thermocellum Dihydrodipicolinate synthase (dapA) CSB-YP386377DUT
CSB-EP386377DUT
CSB-BP386377DUT
CSB-MP386377DUT
CSB-EP386377DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Protein MraZ (mraZ) CSB-YP386378DUT
CSB-EP386378DUT
CSB-BP386378DUT
CSB-MP386378DUT
CSB-EP386378DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Ribosome maturation factor RimP (rimP) CSB-YP386379DUT
CSB-EP386379DUT
CSB-BP386379DUT
CSB-MP386379DUT
CSB-EP386379DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Uridylate kinase (pyrH) CSB-YP386380DUT
CSB-EP386380DUT
CSB-BP386380DUT
CSB-MP386380DUT
CSB-EP386380DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum DNA ligase (ligA), partial CSB-YP386381DUT
CSB-EP386381DUT
CSB-BP386381DUT
CSB-MP386381DUT
CSB-EP386381DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Probable tRNA sulfurtransferase (thiI) CSB-YP386382DUT
CSB-EP386382DUT
CSB-BP386382DUT
CSB-MP386382DUT
CSB-EP386382DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum DNA repair protein recO (recO) CSB-YP386383DUT
CSB-EP386383DUT
CSB-BP386383DUT
CSB-MP386383DUT
CSB-EP386383DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Small, acid-soluble spore protein H (sspH) CSB-YP386384DUT
CSB-EP386384DUT
CSB-BP386384DUT
CSB-MP386384DUT
CSB-EP386384DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum 50S ribosomal protein L35 (rpmI) CSB-YP386385DUT
CSB-EP386385DUT
CSB-BP386385DUT
CSB-MP386385DUT
CSB-EP386385DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Leucine--tRNA ligase (leuS), partial CSB-YP386386DUT
CSB-EP386386DUT
CSB-BP386386DUT
CSB-MP386386DUT
CSB-EP386386DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum 50S ribosomal protein L28 (rpmB) CSB-YP386387DUT
CSB-EP386387DUT
CSB-BP386387DUT
CSB-MP386387DUT
CSB-EP386387DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Glycine--tRNA ligase (glyQS) CSB-YP386388DUT
CSB-EP386388DUT
CSB-BP386388DUT
CSB-MP386388DUT
CSB-EP386388DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Chaperone protein DnaJ (dnaJ) CSB-YP386389DUT
CSB-EP386389DUT
CSB-BP386389DUT
CSB-MP386389DUT
CSB-EP386389DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Elongation factor 4 (lepA), partial CSB-YP386390DUT
CSB-EP386390DUT
CSB-BP386390DUT
CSB-MP386390DUT
CSB-EP386390DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Protein translocase subunit SecA (secA), partial CSB-YP386391DUT
CSB-EP386391DUT
CSB-BP386391DUT
CSB-MP386391DUT
CSB-EP386391DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum UPF0059 membrane protein Cthe_1420 (Cthe_1420), partial CSB-YP386392DUT1
CSB-EP386392DUT1
CSB-BP386392DUT1
CSB-MP386392DUT1
CSB-EP386392DUT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Urease accessory protein UreD (ureD) CSB-YP386393DUT
CSB-EP386393DUT
CSB-BP386393DUT
CSB-MP386393DUT
CSB-EP386393DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Urease subunit alpha (ureC), partial CSB-YP386394DUT
CSB-EP386394DUT
CSB-BP386394DUT
CSB-MP386394DUT
CSB-EP386394DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum 33 kDa chaperonin (hslO) CSB-YP386395DUT
CSB-EP386395DUT
CSB-BP386395DUT
CSB-MP386395DUT
CSB-EP386395DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Acetylglutamate kinase (argB) CSB-YP386396DUT
CSB-EP386396DUT
CSB-BP386396DUT
CSB-MP386396DUT
CSB-EP386396DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.jhhuazhong.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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