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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Marinobacter aquaeolei Ribonuclease P protein component (rnpA) CSB-YP378259MNI
CSB-EP378259MNI
CSB-BP378259MNI
CSB-MP378259MNI
CSB-EP378259MNI-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP378260MOM
CSB-EP378260MOM
CSB-BP378260MOM
CSB-MP378260MOM
CSB-EP378260MOM-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Chromosomal replication initiator protein DnaA (dnaA) CSB-YP378261MOM
CSB-EP378261MOM
CSB-BP378261MOM
CSB-MP378261MOM
CSB-EP378261MOM-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. UPF0233 membrane protein Mkms_0020 (Mkms_0020), partial CSB-YP378262MOM1
CSB-EP378262MOM1
CSB-BP378262MOM1
CSB-MP378262MOM1
CSB-EP378262MOM1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Putative S-adenosyl-L-methionine-dependent methyltransferase Mkms_0098 (Mkms_0098) CSB-YP378263MOM
CSB-EP378263MOM
CSB-BP378263MOM
CSB-MP378263MOM
CSB-EP378263MOM-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Putative S-adenosyl-L-methionine-dependent methyltransferase Mkms_0379 (Mkms_0379) CSB-YP378264MOM
CSB-EP378264MOM
CSB-BP378264MOM
CSB-MP378264MOM
CSB-EP378264MOM-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Deoxycytidine triphosphate deaminase (dcd) CSB-YP378265MOM
CSB-EP378265MOM
CSB-BP378265MOM
CSB-MP378265MOM
CSB-EP378265MOM-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Na (+)/H (+) antiporter nhaA 1, partial CSB-YP378266MOM
CSB-EP378266MOM
CSB-BP378266MOM
CSB-MP378266MOM
CSB-EP378266MOM-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Acetate kinase (ackA) CSB-YP378267MOM
CSB-EP378267MOM
CSB-BP378267MOM
CSB-MP378267MOM
CSB-EP378267MOM-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Putative S-adenosyl-L-methionine-dependent methyltransferase Mkms_0592 (Mkms_0592) CSB-YP378268MOM
CSB-EP378268MOM
CSB-BP378268MOM
CSB-MP378268MOM
CSB-EP378268MOM-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. 60 kDa chaperonin 1 (groL1), partial CSB-YP378269MOM
CSB-EP378269MOM
CSB-BP378269MOM
CSB-MP378269MOM
CSB-EP378269MOM-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP378270MOM
CSB-EP378270MOM
CSB-BP378270MOM
CSB-MP378270MOM
CSB-EP378270MOM-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Demethylmenaquinone methyltransferase (ubiE) CSB-YP378271MOM
CSB-EP378271MOM
CSB-BP378271MOM
CSB-MP378271MOM
CSB-EP378271MOM-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. 50S ribosomal protein L33 1 (rpmG1) CSB-YP378272MOM
CSB-EP378272MOM
CSB-BP378272MOM
CSB-MP378272MOM
CSB-EP378272MOM-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. 50S ribosomal protein L1 (rplA) CSB-YP378273MOM
CSB-EP378273MOM
CSB-BP378273MOM
CSB-MP378273MOM
CSB-EP378273MOM-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. 50S ribosomal protein L10 (rplJ) CSB-YP378274MOM
CSB-EP378274MOM
CSB-BP378274MOM
CSB-MP378274MOM
CSB-EP378274MOM-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP378275MOM
CSB-EP378275MOM
CSB-BP378275MOM
CSB-MP378275MOM
CSB-EP378275MOM-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. 30S ribosomal protein S12 (rpsL) CSB-YP378276MOM
CSB-EP378276MOM
CSB-BP378276MOM
CSB-MP378276MOM
CSB-EP378276MOM-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. 50S ribosomal protein L3 (rplC) CSB-YP378277MOM
CSB-EP378277MOM
CSB-BP378277MOM
CSB-MP378277MOM
CSB-EP378277MOM-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. 50S ribosomal protein L4 (rplD) CSB-YP378278MOM
CSB-EP378278MOM
CSB-BP378278MOM
CSB-MP378278MOM
CSB-EP378278MOM-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中，除了表達系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示，可以幫助你做出明智的決策。

第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條：如何選擇合適的表達載體?

表達載體是一種DNA分子，它攜帶特定的基因進入宿主細胞，并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達，經(jīng)過多年的發(fā)展，表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示，本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條：如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用，對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.jhhuazhong.com/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達服務，您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問，涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等，您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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