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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Burkholderia mallei D-alanine--D-alanine ligase (ddl) CSB-YP379660BPR
CSB-EP379660BPR
CSB-BP379660BPR
CSB-MP379660BPR
CSB-EP379660BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP379661BPR
CSB-EP379661BPR
CSB-BP379661BPR
CSB-MP379661BPR
CSB-EP379661BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Protein MraZ (mraZ) CSB-YP379662BPR
CSB-EP379662BPR
CSB-BP379662BPR
CSB-MP379662BPR
CSB-EP379662BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Chaperone protein DnaK (dnaK), partial CSB-YP379663BPR
CSB-EP379663BPR
CSB-BP379663BPR
CSB-MP379663BPR
CSB-EP379663BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei 3-deoxy-manno-octulosonate cytidylyltransferase (kdsB) CSB-YP379664BPR
CSB-EP379664BPR
CSB-BP379664BPR
CSB-MP379664BPR
CSB-EP379664BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei ATP-dependent Clp protease adapter protein ClpS (clpS) CSB-YP379665BPR
CSB-EP379665BPR
CSB-BP379665BPR
CSB-MP379665BPR
CSB-EP379665BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei UPF0234 protein BMASAVP1_A0673 (BMASAVP1_A0673) CSB-YP379666BPR
CSB-EP379666BPR
CSB-BP379666BPR
CSB-MP379666BPR
CSB-EP379666BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Urease accessory protein UreG (ureG) CSB-YP379667BPR
CSB-EP379667BPR
CSB-BP379667BPR
CSB-MP379667BPR
CSB-EP379667BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Urease accessory protein UreE (ureE) CSB-YP379668BPR
CSB-EP379668BPR
CSB-BP379668BPR
CSB-MP379668BPR
CSB-EP379668BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Urease accessory protein UreD (ureD) CSB-YP379669BPR
CSB-EP379669BPR
CSB-BP379669BPR
CSB-MP379669BPR
CSB-EP379669BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Undecaprenyl-diphosphatase (uppP), partial CSB-YP379670BPR1
CSB-EP379670BPR1
CSB-BP379670BPR1
CSB-MP379670BPR1
CSB-EP379670BPR1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP379671BPR
CSB-EP379671BPR
CSB-BP379671BPR
CSB-MP379671BPR
CSB-EP379671BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Transcriptional repressor NrdR (nrdR) CSB-YP379672BPR
CSB-EP379672BPR
CSB-BP379672BPR
CSB-MP379672BPR
CSB-EP379672BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei 60 kDa chaperonin (groL), partial CSB-YP379673BPR
CSB-EP379673BPR
CSB-BP379673BPR
CSB-MP379673BPR
CSB-EP379673BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Aspartate carbamoyltransferase (pyrB) CSB-YP379674BPR
CSB-EP379674BPR
CSB-BP379674BPR
CSB-MP379674BPR
CSB-EP379674BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei UPF0176 protein BMASAVP1_A1042 (BMASAVP1_A1042) CSB-YP379675BPR
CSB-EP379675BPR
CSB-BP379675BPR
CSB-MP379675BPR
CSB-EP379675BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Ketol-acid reductoisomerase (ilvC) CSB-YP379676BPR
CSB-EP379676BPR
CSB-BP379676BPR
CSB-MP379676BPR
CSB-EP379676BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei NADH-quinone oxidoreductase subunit A (nuoA), partial CSB-YP379677BPR1
CSB-EP379677BPR1
CSB-BP379677BPR1
CSB-MP379677BPR1
CSB-EP379677BPR1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei UPF0246 protein BMASAVP1_A1161 (BMASAVP1_A1161) CSB-YP379678BPR
CSB-EP379678BPR
CSB-BP379678BPR
CSB-MP379678BPR
CSB-EP379678BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei UPF0060 membrane protein BMASAVP1_A1271 (BMASAVP1_A1271), partial CSB-YP379679BPR1
CSB-EP379679BPR1
CSB-BP379679BPR1
CSB-MP379679BPR1
CSB-EP379679BPR1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產(chǎn)生大量蛋白質、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產(chǎn)生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.jhhuazhong.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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