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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus halodurans UDP-N-acetylglucosamine 1-carboxyvinyltransferase 1 (murA1) CSB-YP881477BQS
CSB-EP881477BQS
CSB-BP881477BQS
CSB-MP881477BQS
CSB-EP881477BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans Flagellar hook-associated protein 2 (fliD), partial CSB-YP881478BQS
CSB-EP881478BQS
CSB-BP881478BQS
CSB-MP881478BQS
CSB-EP881478BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans Histidinol dehydrogenase (hisD) CSB-YP881479BQS
CSB-EP881479BQS
CSB-BP881479BQS
CSB-MP881479BQS
CSB-EP881479BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans UPF0042 nucleotide-binding protein BH3569 (BH3569) CSB-YP881480BQS
CSB-EP881480BQS
CSB-BP881480BQS
CSB-MP881480BQS
CSB-EP881480BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI), partial CSB-YP881481BQS
CSB-EP881481BQS
CSB-BP881481BQS
CSB-MP881481BQS
CSB-EP881481BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans Putative AgrB-like protein (BH3475), partial CSB-YP881482BQS1
CSB-EP881482BQS1
CSB-BP881482BQS1
CSB-MP881482BQS1
CSB-EP881482BQS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans Diaminopimelate epimerase (dapF) CSB-YP881483BQS
CSB-EP881483BQS
CSB-BP881483BQS
CSB-MP881483BQS
CSB-EP881483BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans S-adenosylmethionine synthase (metK) CSB-YP881484BQS
CSB-EP881484BQS
CSB-BP881484BQS
CSB-MP881484BQS
CSB-EP881484BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans UPF0354 protein BH3252 (BH3252) CSB-YP881485BQS
CSB-EP881485BQS
CSB-BP881485BQS
CSB-MP881485BQS
CSB-EP881485BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans Argininosuccinate synthase (argG) CSB-YP881486BQS
CSB-EP881486BQS
CSB-BP881486BQS
CSB-MP881486BQS
CSB-EP881486BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans Translation initiation factor IF-3 (infC) CSB-YP881487BQS
CSB-EP881487BQS
CSB-BP881487BQS
CSB-MP881487BQS
CSB-EP881487BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans UvrABC system protein C (uvrC), partial CSB-YP881488BQS
CSB-EP881488BQS
CSB-BP881488BQS
CSB-MP881488BQS
CSB-EP881488BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans Non-canonical purine NTP pyrophosphatase (BH3067) CSB-YP881489BQS
CSB-EP881489BQS
CSB-BP881489BQS
CSB-MP881489BQS
CSB-EP881489BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans 2-isopropylmalate synthase (leuA), partial CSB-YP881490BQS
CSB-EP881490BQS
CSB-BP881490BQS
CSB-MP881490BQS
CSB-EP881490BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans Molybdenum cofactor biosynthesis protein C (moaC) CSB-YP881491BQS
CSB-EP881491BQS
CSB-BP881491BQS
CSB-MP881491BQS
CSB-EP881491BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans Protein CrcB homolog 2 (crcB2), partial CSB-YP881492BQS1
CSB-EP881492BQS1
CSB-BP881492BQS1
CSB-MP881492BQS1
CSB-EP881492BQS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans N-acetyl-gamma-glutamyl-phosphate reductase (argC) CSB-YP881493BQS
CSB-EP881493BQS
CSB-BP881493BQS
CSB-MP881493BQS
CSB-EP881493BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans Pantothenate kinase (coaA) CSB-YP881494BQS
CSB-EP881494BQS
CSB-BP881494BQS
CSB-MP881494BQS
CSB-EP881494BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans Adapter protein mecA 2 (mecA2) CSB-YP881495BQS
CSB-EP881495BQS
CSB-BP881495BQS
CSB-MP881495BQS
CSB-EP881495BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans Bis (5'-nucleosyl)-tetraphosphatase prpE [asymmetrical] (prpE) CSB-YP881496BQS
CSB-EP881496BQS
CSB-BP881496BQS
CSB-MP881496BQS
CSB-EP881496BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.jhhuazhong.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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