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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Arabidopsis thaliana Protein LURP-one-related 15 (At5g01750) CSB-YP881877DOA
CSB-EP881877DOA
CSB-BP881877DOA
CSB-MP881877DOA
CSB-EP881877DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Spindle and kinetochore-associated protein 1 homolog (At3g60660) CSB-YP881878DOA
CSB-EP881878DOA
CSB-BP881878DOA
CSB-MP881878DOA
CSB-EP881878DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Thylakoid ADP,ATP carrier protein, chloroplastic (TAAC), partial CSB-YP881879DOA1
CSB-EP881879DOA1
CSB-BP881879DOA1
CSB-MP881879DOA1
CSB-EP881879DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At4g36680, mitochondrial (At4g36680) CSB-YP881880DOA
CSB-EP881880DOA
CSB-BP881880DOA
CSB-MP881880DOA
CSB-EP881880DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana DDB1- and CUL4-associated factor homolog 1 (DCAF1), partial CSB-YP881881DOA
CSB-EP881881DOA
CSB-BP881881DOA
CSB-MP881881DOA
CSB-EP881881DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative EG45-like domain containing protein 1 (EGC1) CSB-YP881882DOA
CSB-EP881882DOA
CSB-BP881882DOA
CSB-MP881882DOA
CSB-EP881882DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Defensin-like protein 161 (LCR27) CSB-YP881883DOA
CSB-EP881883DOA
CSB-BP881883DOA
CSB-MP881883DOA
CSB-EP881883DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Transcription factor LAF1 (LAF1) CSB-YP881884DOA
CSB-EP881884DOA
CSB-BP881884DOA
CSB-MP881884DOA
CSB-EP881884DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana UDP-glycosyltransferase 79B7 (UGT79B7) CSB-YP881885DOA
CSB-EP881885DOA
CSB-BP881885DOA
CSB-MP881885DOA
CSB-EP881885DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana RING-H2 finger protein ATL39 (ATL39), partial CSB-YP881886DOA1
CSB-EP881886DOA1
CSB-BP881886DOA1
CSB-MP881886DOA1
CSB-EP881886DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative F-box protein At4g05475 (At4g05475) CSB-YP881887DOA
CSB-EP881887DOA
CSB-BP881887DOA
CSB-MP881887DOA
CSB-EP881887DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana 4-coumarate--CoA ligase-like 7 (4CLL7), partial CSB-YP881888DOA
CSB-EP881888DOA
CSB-BP881888DOA
CSB-MP881888DOA
CSB-EP881888DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Calcium-dependent protein kinase 23 (CPK23), partial CSB-YP881889DOA
CSB-EP881889DOA
CSB-BP881889DOA
CSB-MP881889DOA
CSB-EP881889DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Rhodanese-like domain-containing protein 4, chloroplastic (STR4), partial CSB-YP881890DOA1
CSB-EP881890DOA1
CSB-BP881890DOA1
CSB-MP881890DOA1
CSB-EP881890DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable nitrate excretion transporter 3 (At3g45680), partial CSB-YP881891DOA
CSB-EP881891DOA
CSB-BP881891DOA
CSB-MP881891DOA
CSB-EP881891DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At3g60050 (At3g60050) CSB-YP881892DOA
CSB-EP881892DOA
CSB-BP881892DOA
CSB-MP881892DOA
CSB-EP881892DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Basic leucine zipper 25 (BZIP25) CSB-YP881893DOA
CSB-EP881893DOA
CSB-BP881893DOA
CSB-MP881893DOA
CSB-EP881893DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Transcription factor ORG2 (ORG2) CSB-YP881894DOA
CSB-EP881894DOA
CSB-BP881894DOA
CSB-MP881894DOA
CSB-EP881894DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Auxin-responsive protein IAA30 (IAA30) CSB-YP881895DOA
CSB-EP881895DOA
CSB-BP881895DOA
CSB-MP881895DOA
CSB-EP881895DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Defensin-like protein 11 (At3g63360) CSB-YP881896DOA
CSB-EP881896DOA
CSB-BP881896DOA
CSB-MP881896DOA
CSB-EP881896DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.jhhuazhong.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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