重組蛋白
重組蛋白質(zhì)是分析生物相互作用的有用工具。在研究實(shí)驗(yàn)室中使用了大量的重組蛋白質(zhì)，包括商業(yè)蛋白質(zhì)和在特定研究項(xiàng)目中生成的蛋白質(zhì)。它們?cè)谠S多應(yīng)用中發(fā)揮著關(guān)鍵作用。
如果您正在尋找具有優(yōu)良質(zhì)量（驗(yàn)證的生物活性，較低的內(nèi)毒素水平和高純度）的重組蛋白質(zhì)來支持您的研究，您來對(duì)地方了。
經(jīng)過18年的發(fā)展，華美生物已經(jīng)建立了從原核到真核的五大系統(tǒng)，用于表達(dá)重組蛋白質(zhì)。我們擁有一系列蛋白質(zhì)表達(dá)和純化技術(shù)，包括基于桿狀病毒外膜蛋白（OMP）的蛋白質(zhì)表達(dá)技術(shù)，以及膜蛋白表達(dá)技術(shù)等。
產(chǎn)品特點(diǎn)
- 超過13,000種重組蛋白覆蓋大多數(shù)基因/靶點(diǎn)；
- 5種表達(dá)系統(tǒng)，包括大腸桿菌（E.coli）、酵母、桿狀病毒感染的昆蟲細(xì)胞、哺乳動(dòng)物細(xì)胞和體外大腸桿菌。而體外大腸桿菌表達(dá)系統(tǒng)是華美生物的獨(dú)特表達(dá)系統(tǒng)。與傳統(tǒng)蛋白表達(dá)系統(tǒng)相比，體外大腸桿菌省略了許多過程，如質(zhì)粒轉(zhuǎn)化、細(xì)胞培養(yǎng)、收集、破碎和離心等，大大提高了工作效率。
- 多種標(biāo)簽，包括His、GST、Flag、MBP等。
- 廣泛應(yīng)用，包括抗原、細(xì)胞分析、結(jié)合分析/蛋白質(zhì)相互作用、細(xì)胞培養(yǎng)-無血清培養(yǎng)基、藥物相關(guān)研究、體外酶活性、ELISA標(biāo)準(zhǔn)及其原料、體內(nèi)研究、質(zhì)譜標(biāo)準(zhǔn)、蛋白芯片、SDS-PAGE控制、蛋白質(zhì)結(jié)構(gòu)分析（晶體/電子顯微鏡）。
- 多種物種，包括人類、小鼠、大鼠、兔子、豚鼠、雞、猴子、豬等。
- 無動(dòng)物副產(chǎn)品的動(dòng)物源性重組蛋白質(zhì)。華美生物已開發(fā)出無動(dòng)物副產(chǎn)品接觸的重組蛋白質(zhì)產(chǎn)品線。
重組蛋白目錄
以下是所有重組蛋白的目錄。您可以在目錄中找到適合您研究的蛋白質(zhì)，或在本網(wǎng)站頭部的搜索框中搜索它們。
如果您找不到您需要的抗體，請(qǐng)查看華美生物蛋白質(zhì)定制服務(wù)。
通過選擇目標(biāo)名稱的首字母瀏覽產(chǎn)品。
| 產(chǎn)品名稱 | 貨號(hào) | 規(guī)格 |
|---|---|---|
| Recombinant Geobacillus kaustophilus 3-dehydroquinate dehydratase (aroD) | CSB-YP708536GAAA CSB-EP708536GAAA CSB-BP708536GAAA CSB-MP708536GAAA CSB-EP708536GAAA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Geobacillus kaustophilus Dihydroxy-acid dehydratase (ilvD), partial | CSB-YP708537GAAA CSB-EP708537GAAA CSB-BP708537GAAA CSB-MP708537GAAA CSB-EP708537GAAA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Geobacillus kaustophilus Ribulokinase (araB), partial | CSB-YP708538GAAA CSB-EP708538GAAA CSB-BP708538GAAA CSB-MP708538GAAA CSB-EP708538GAAA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Geobacillus kaustophilus Glycerol-3-phosphate acyltransferase (plsY), partial | CSB-YP708539GAAA1 CSB-EP708539GAAA1 CSB-BP708539GAAA1 CSB-MP708539GAAA1 CSB-EP708539GAAA1-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Geobacillus kaustophilus Purine nucleoside phosphorylase DeoD-type (deoD) | CSB-YP708540GAAA CSB-EP708540GAAA CSB-BP708540GAAA CSB-MP708540GAAA CSB-EP708540GAAA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Geobacillus kaustophilus Thiamine-phosphate synthase (thiE) | CSB-YP708541GAAA CSB-EP708541GAAA CSB-BP708541GAAA CSB-MP708541GAAA CSB-EP708541GAAA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Geobacillus kaustophilus Sulfite reductase [NADPH] hemoprotein beta-component (cysI), partial | CSB-YP708542GAAA CSB-EP708542GAAA CSB-BP708542GAAA CSB-MP708542GAAA CSB-EP708542GAAA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Geobacillus kaustophilus Urocanate hydratase (hutU), partial | CSB-YP708543GAAA CSB-EP708543GAAA CSB-BP708543GAAA CSB-MP708543GAAA CSB-EP708543GAAA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Geobacillus kaustophilus Small, acid-soluble spore protein O (sspO) | CSB-YP708544GAAA CSB-EP708544GAAA CSB-BP708544GAAA CSB-MP708544GAAA CSB-EP708544GAAA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Geobacillus kaustophilus DNA mismatch repair protein mutL (mutL), partial | CSB-YP708545GAAA CSB-EP708545GAAA CSB-BP708545GAAA CSB-MP708545GAAA CSB-EP708545GAAA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Geobacillus kaustophilus Undecaprenyl pyrophosphate synthase (uppS) | CSB-YP708546GAAA CSB-EP708546GAAA CSB-BP708546GAAA CSB-MP708546GAAA CSB-EP708546GAAA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Geobacillus kaustophilus Probable chemoreceptor glutamine deamidase CheD (cheD) | CSB-YP708547GAAA CSB-EP708547GAAA CSB-BP708547GAAA CSB-MP708547GAAA CSB-EP708547GAAA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Geobacillus kaustophilus 50S ribosomal protein L19 (rplS) | CSB-YP708548GAAA CSB-EP708548GAAA CSB-BP708548GAAA CSB-MP708548GAAA CSB-EP708548GAAA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Geobacillus kaustophilus UPF0122 protein GK1195 (GK1195) | CSB-YP708549GAAA CSB-EP708549GAAA CSB-BP708549GAAA CSB-MP708549GAAA CSB-EP708549GAAA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Geobacillus kaustophilus Dihydroorotate dehydrogenase B (NAD (+)), catalytic subunit (pyrD) | CSB-YP708550GAAA CSB-EP708550GAAA CSB-BP708550GAAA CSB-MP708550GAAA CSB-EP708550GAAA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Geobacillus kaustophilus UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase (murE) | CSB-YP708551GAAA CSB-EP708551GAAA CSB-BP708551GAAA CSB-MP708551GAAA CSB-EP708551GAAA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Geobacillus kaustophilus Phosphopantetheine adenylyltransferase (coaD) | CSB-YP708552GAAA CSB-EP708552GAAA CSB-BP708552GAAA CSB-MP708552GAAA CSB-EP708552GAAA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Geobacillus kaustophilus UPF0356 protein GK1054 (GK1054) | CSB-YP708553GAAA CSB-EP708553GAAA CSB-BP708553GAAA CSB-MP708553GAAA CSB-EP708553GAAA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Geobacillus kaustophilus 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate phosphatase (mtnX) | CSB-YP708554GAAA CSB-EP708554GAAA CSB-BP708554GAAA CSB-MP708554GAAA CSB-EP708554GAAA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Geobacillus kaustophilus Acetylglutamate kinase (argB) | CSB-YP708555GAAA CSB-EP708555GAAA CSB-BP708555GAAA CSB-MP708555GAAA CSB-EP708555GAAA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
有用資源
什么是重組蛋白?
重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中，除了表達(dá)系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí)，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示，可以幫助你做出明智的決策。
第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。
第二條：如何選擇合適的表達(dá)載體?
表達(dá)載體是一種DNA分子，它攜帶特定的基因進(jìn)入宿主細(xì)胞，并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá)，經(jīng)過多年的發(fā)展，表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示，本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。
根據(jù)重組蛋白的不同應(yīng)用，對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.jhhuazhong.com/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達(dá)服務(wù)，您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問，涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等，您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。
引用資料
Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023
Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023
Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023
Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023
Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022
