重組蛋白
重組蛋白質(zhì)是分析生物相互作用的有用工具。在研究實驗室中使用了大量的重組蛋白質(zhì)，包括商業(yè)蛋白質(zhì)和在特定研究項目中生成的蛋白質(zhì)。它們在許多應(yīng)用中發(fā)揮著關(guān)鍵作用。
如果您正在尋找具有優(yōu)良質(zhì)量（驗證的生物活性，較低的內(nèi)毒素水平和高純度）的重組蛋白質(zhì)來支持您的研究，您來對地方了。
經(jīng)過18年的發(fā)展，華美生物已經(jīng)建立了從原核到真核的五大系統(tǒng)，用于表達重組蛋白質(zhì)。我們擁有一系列蛋白質(zhì)表達和純化技術(shù)，包括基于桿狀病毒外膜蛋白（OMP）的蛋白質(zhì)表達技術(shù)，以及膜蛋白表達技術(shù)等。
產(chǎn)品特點
- 超過13,000種重組蛋白覆蓋大多數(shù)基因/靶點；
- 5種表達系統(tǒng)，包括大腸桿菌（E.coli）、酵母、桿狀病毒感染的昆蟲細胞、哺乳動物細胞和體外大腸桿菌。而體外大腸桿菌表達系統(tǒng)是華美生物的獨特表達系統(tǒng)。與傳統(tǒng)蛋白表達系統(tǒng)相比，體外大腸桿菌省略了許多過程，如質(zhì)粒轉(zhuǎn)化、細胞培養(yǎng)、收集、破碎和離心等，大大提高了工作效率。
- 多種標簽，包括His、GST、Flag、MBP等。
- 廣泛應(yīng)用，包括抗原、細胞分析、結(jié)合分析/蛋白質(zhì)相互作用、細胞培養(yǎng)-無血清培養(yǎng)基、藥物相關(guān)研究、體外酶活性、ELISA標準及其原料、體內(nèi)研究、質(zhì)譜標準、蛋白芯片、SDS-PAGE控制、蛋白質(zhì)結(jié)構(gòu)分析（晶體/電子顯微鏡）。
- 多種物種，包括人類、小鼠、大鼠、兔子、豚鼠、雞、猴子、豬等。
- 無動物副產(chǎn)品的動物源性重組蛋白質(zhì)。華美生物已開發(fā)出無動物副產(chǎn)品接觸的重組蛋白質(zhì)產(chǎn)品線。
重組蛋白目錄
以下是所有重組蛋白的目錄。您可以在目錄中找到適合您研究的蛋白質(zhì)，或在本網(wǎng)站頭部的搜索框中搜索它們。
如果您找不到您需要的抗體，請查看華美生物蛋白質(zhì)定制服務(wù)。
通過選擇目標名稱的首字母瀏覽產(chǎn)品。
| 產(chǎn)品名稱 | 貨號 | 規(guī)格 |
|---|---|---|
| Recombinant Methanocaldococcus jannaschii Putative hydrogenase maturation protease MJ0253 (MJ0253) | CSB-YP710137MRU CSB-EP710137MRU CSB-BP710137MRU CSB-MP710137MRU CSB-EP710137MRU-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Methanocaldococcus jannaschii Uncharacterized protein MJ0260 (MJ0260), partial | CSB-YP710138MRU1 CSB-EP710138MRU1 CSB-BP710138MRU1 CSB-MP710138MRU1 CSB-EP710138MRU1-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Methanocaldococcus jannaschii Pyruvate synthase subunit porA (porA) | CSB-YP710139MRU CSB-EP710139MRU CSB-BP710139MRU CSB-MP710139MRU CSB-EP710139MRU-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Methanocaldococcus jannaschii Uncharacterized HTH-type transcriptional regulator MJ0272 (MJ0272) | CSB-YP710140MRU CSB-EP710140MRU CSB-BP710140MRU CSB-MP710140MRU CSB-EP710140MRU-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Methanocaldococcus jannaschii Nascent polypeptide-associated complex protein (nac) | CSB-YP710141MRU CSB-EP710141MRU CSB-BP710141MRU CSB-MP710141MRU CSB-EP710141MRU-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Methanocaldococcus jannaschii Small heat shock protein HSP16.5 (MJ0285) | CSB-YP710142MRU CSB-EP710142MRU CSB-BP710142MRU CSB-MP710142MRU CSB-EP710142MRU-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Methanocaldococcus jannaschii Uncharacterized protein MJ0290 (MJ0290) | CSB-YP710143MRU CSB-EP710143MRU CSB-BP710143MRU CSB-MP710143MRU CSB-EP710143MRU-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Methanocaldococcus jannaschii Uncharacterized ATP-dependent helicase MJ0294 (MJ0294), partial | CSB-YP710144MRU CSB-EP710144MRU CSB-BP710144MRU CSB-MP710144MRU CSB-EP710144MRU-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Methanocaldococcus jannaschii Elongation factor 1-alpha (tuf) | CSB-YP710145MRU CSB-EP710145MRU CSB-BP710145MRU CSB-MP710145MRU CSB-EP710145MRU-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Methanocaldococcus jannaschii Uncharacterized protein MJ0329 (MJ0329), partial | CSB-YP710146MRU CSB-EP710146MRU CSB-BP710146MRU CSB-MP710146MRU CSB-EP710146MRU-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Methanocaldococcus jannaschii Uncharacterized protein MJ0337 (MJ0337) | CSB-YP710147MRU CSB-EP710147MRU CSB-BP710147MRU CSB-MP710147MRU CSB-EP710147MRU-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Methanocaldococcus jannaschii Uncharacterized protein MJ0343 (MJ0343), partial | CSB-YP710148MRU CSB-EP710148MRU CSB-BP710148MRU CSB-MP710148MRU CSB-EP710148MRU-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Methanocaldococcus jannaschii Uncharacterized protein MJ0348 (MJ0348), partial | CSB-YP710149MRU CSB-EP710149MRU CSB-BP710149MRU CSB-MP710149MRU CSB-EP710149MRU-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Brucella abortus biovar 1 Leu/Ile/Val-binding protein homolog 7 (BruAb2_0801) | CSB-YP710150BWS CSB-EP710150BWS CSB-BP710150BWS CSB-MP710150BWS CSB-EP710150BWS-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Brucella abortus biovar 1 ATP-dependent dethiobiotin synthetase BioD (bioD) | CSB-YP710151BWS CSB-EP710151BWS CSB-BP710151BWS CSB-MP710151BWS CSB-EP710151BWS-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Brucella abortus biovar 1 UPF0173 metal-dependent hydrolase BruAb2_0628 (BruAb2_0628) | CSB-YP710152BWS CSB-EP710152BWS CSB-BP710152BWS CSB-MP710152BWS CSB-EP710152BWS-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Methanocaldococcus jannaschii Uncharacterized protein MJ0357 (MJ0357) | CSB-YP710153MRU CSB-EP710153MRU CSB-BP710153MRU CSB-MP710153MRU CSB-EP710153MRU-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Methanocaldococcus jannaschii Uncharacterized protein MJ0364 (MJ0364) | CSB-YP710154MRU CSB-EP710154MRU CSB-BP710154MRU CSB-MP710154MRU CSB-EP710154MRU-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Methanocaldococcus jannaschii Preprotein translocase subunit SecE (secE), partial | CSB-YP710155MRU CSB-EP710155MRU CSB-BP710155MRU CSB-MP710155MRU CSB-EP710155MRU-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Methanocaldococcus jannaschii Uncharacterized protein MJ0381 (MJ0381) | CSB-YP710156MRU CSB-EP710156MRU CSB-BP710156MRU CSB-MP710156MRU CSB-EP710156MRU-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
有用資源
什么是重組蛋白?
重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中，除了表達系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示，可以幫助你做出明智的決策。
第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。
第二條：如何選擇合適的表達載體?
表達載體是一種DNA分子，它攜帶特定的基因進入宿主細胞，并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達，經(jīng)過多年的發(fā)展，表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示，本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。
根據(jù)重組蛋白的不同應(yīng)用，對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.jhhuazhong.com/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達服務(wù)，您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問，涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等，您可以點擊橙色按鈕為我們在線留言。
引用資料
Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023
Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023
Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023
Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023
Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022
