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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia coli UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase CSB-YP511485ENU
CSB-EP511485ENU
CSB-BP511485ENU
CSB-MP511485ENU
CSB-EP511485ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UPF0231 protein yacL (yacL) CSB-YP511486ENU
CSB-EP511486ENU
CSB-BP511486ENU
CSB-MP511486ENU
CSB-EP511486ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Sugar fermentation stimulation protein A (sfsA) CSB-YP511487ENU
CSB-EP511487ENU
CSB-BP511487ENU
CSB-MP511487ENU
CSB-EP511487ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli [Protein-PII] uridylyltransferase (glnD), partial CSB-YP511488ENU
CSB-EP511488ENU
CSB-BP511488ENU
CSB-MP511488ENU
CSB-EP511488ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP511489ENU
CSB-EP511489ENU
CSB-BP511489ENU
CSB-MP511489ENU
CSB-EP511489ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Glucans biosynthesis protein G (mdoG) CSB-YP511490ENU
CSB-EP511490ENU
CSB-BP511490ENU
CSB-MP511490ENU
CSB-EP511490ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Flagellar P-ring protein (flgI) CSB-YP511491ENU
CSB-EP511491ENU
CSB-BP511491ENU
CSB-MP511491ENU
CSB-EP511491ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Peptidase T (pepT) CSB-YP511492ENU
CSB-EP511492ENU
CSB-BP511492ENU
CSB-MP511492ENU
CSB-EP511492ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Histidinol-phosphate aminotransferase (hisC) CSB-YP511493ENU
CSB-EP511493ENU
CSB-BP511493ENU
CSB-MP511493ENU
CSB-EP511493ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Methionine--tRNA ligase (metG) CSB-YP511494ENU
CSB-EP511494ENU
CSB-BP511494ENU
CSB-MP511494ENU
CSB-EP511494ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UPF0102 protein yraN (yraN) CSB-YP511495ENU
CSB-EP511495ENU
CSB-BP511495ENU
CSB-MP511495ENU
CSB-EP511495ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Phosphoglucosamine mutase (glmM) CSB-YP511496ENU
CSB-EP511496ENU
CSB-BP511496ENU
CSB-MP511496ENU
CSB-EP511496ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Monofunctional biosynthetic peptidoglycan transglycosylase (mtgA), partial CSB-YP511497ENU1
CSB-EP511497ENU1
CSB-BP511497ENU1
CSB-MP511497ENU1
CSB-EP511497ENU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli p-hydroxybenzoic acid efflux pump subunit AaeA (aaeA), partial CSB-YP511498ENU1
CSB-EP511498ENU1
CSB-BP511498ENU1
CSB-MP511498ENU1
CSB-EP511498ENU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Phosphoglycerol transferase I (mdoB), partial CSB-YP511499ENU1
CSB-EP511499ENU1
CSB-BP511499ENU1
CSB-MP511499ENU1
CSB-EP511499ENU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Trp operon repressor (trpR) CSB-YP511500ENU
CSB-EP511500ENU
CSB-BP511500ENU
CSB-MP511500ENU
CSB-EP511500ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Gamma-glutamyl phosphate reductase (proA) CSB-YP511501ENU
CSB-EP511501ENU
CSB-BP511501ENU
CSB-MP511501ENU
CSB-EP511501ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Acyl carrier protein phosphodiesterase (acpH) CSB-YP511502ENU
CSB-EP511502ENU
CSB-BP511502ENU
CSB-MP511502ENU
CSB-EP511502ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Exodeoxyribonuclease 7 small subunit (xseB) CSB-YP511503ENU
CSB-EP511503ENU
CSB-BP511503ENU
CSB-MP511503ENU
CSB-EP511503ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Na (+)/H (+) antiporter nhaB, partial CSB-YP511504ENU
CSB-EP511504ENU
CSB-BP511504ENU
CSB-MP511504ENU
CSB-EP511504ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.jhhuazhong.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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