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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Zygosaccharomyces rouxii Altered inheritance of mitochondria protein 44 (AIM44), partial CSB-YP511789ZBL
CSB-EP511789ZBL
CSB-BP511789ZBL
CSB-MP511789ZBL
CSB-EP511789ZBL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zygosaccharomyces rouxii Ribosomal N-lysine methyltransferase 5 (RKM5) CSB-YP511790ZBL
CSB-EP511790ZBL
CSB-BP511790ZBL
CSB-MP511790ZBL
CSB-EP511790ZBL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zygosaccharomyces rouxii Altered inheritance of mitochondria protein 32 (AIM32) CSB-YP511791ZBL
CSB-EP511791ZBL
CSB-BP511791ZBL
CSB-MP511791ZBL
CSB-EP511791ZBL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zygosaccharomyces rouxii Monopolar spindle protein 2 (MPS2), partial CSB-YP511792ZBL1
CSB-EP511792ZBL1
CSB-BP511792ZBL1
CSB-MP511792ZBL1
CSB-EP511792ZBL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zygosaccharomyces rouxii Pheromone-regulated membrane protein 10 (ZYRO0G17116g), partial CSB-YP511793ZBL
CSB-EP511793ZBL
CSB-BP511793ZBL
CSB-MP511793ZBL
CSB-EP511793ZBL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lachancea thermotolerans Transcription activator of gluconeogenesis ERT1 (ERT1) CSB-YP511794LMB
CSB-EP511794LMB
CSB-BP511794LMB
CSB-MP511794LMB
CSB-EP511794LMB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lachancea thermotolerans Nucleolar protein SWM2 (SWM2) CSB-YP511795LMB
CSB-EP511795LMB
CSB-BP511795LMB
CSB-MP511795LMB
CSB-EP511795LMB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lachancea thermotolerans Nuclear rim protein 1 (NUR1), partial CSB-YP511796LMB1
CSB-EP511796LMB1
CSB-BP511796LMB1
CSB-MP511796LMB1
CSB-EP511796LMB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zygosaccharomyces rouxii Glucose transport transcription regulator RGT1 (RGT1), partial CSB-YP511797ZBL
CSB-EP511797ZBL
CSB-BP511797ZBL
CSB-MP511797ZBL
CSB-EP511797ZBL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthroderma otae Aspartic protease PEP1 (PEP1) CSB-YP511798DOP
CSB-EP511798DOP
CSB-BP511798DOP
CSB-MP511798DOP
CSB-EP511798DOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthroderma otae Protein GET1 (GET1), partial CSB-YP511799DOP1
CSB-EP511799DOP1
CSB-BP511799DOP1
CSB-MP511799DOP1
CSB-EP511799DOP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthroderma otae Dipeptidyl-peptidase 5 (DPP5), partial CSB-YP511800DOP
CSB-EP511800DOP
CSB-BP511800DOP
CSB-MP511800DOP
CSB-EP511800DOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthroderma otae Subtilisin-like protease 8 (SUB8) CSB-YP511801DOP
CSB-EP511801DOP
CSB-BP511801DOP
CSB-MP511801DOP
CSB-EP511801DOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthroderma otae Protein SEY1 (SEY1), partial CSB-YP511802DOP
CSB-EP511802DOP
CSB-BP511802DOP
CSB-MP511802DOP
CSB-EP511802DOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthroderma otae Mitochondrial distribution and morphology protein 10 (MDM10) CSB-YP511803DOP
CSB-EP511803DOP
CSB-BP511803DOP
CSB-MP511803DOP
CSB-EP511803DOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthroderma otae Probable E3 ubiquitin ligase complex SCF subunit sconB (sconB), partial CSB-YP511804DOP
CSB-EP511804DOP
CSB-BP511804DOP
CSB-MP511804DOP
CSB-EP511804DOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthroderma otae Subtilisin-like protease 6 (SUB6) CSB-YP511805DOP
CSB-EP511805DOP
CSB-BP511805DOP
CSB-MP511805DOP
CSB-EP511805DOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthroderma otae Vacuolar protease A (PEP2) CSB-YP511806DOP
CSB-EP511806DOP
CSB-BP511806DOP
CSB-MP511806DOP
CSB-EP511806DOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthroderma otae Tripeptidyl-peptidase SED3 (SED3) CSB-YP511807DOP
CSB-EP511807DOP
CSB-BP511807DOP
CSB-MP511807DOP
CSB-EP511807DOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthroderma otae Long chronological lifespan protein 2 (LCL2) CSB-YP511808DOP
CSB-EP511808DOP
CSB-BP511808DOP
CSB-MP511808DOP
CSB-EP511808DOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://www.jhhuazhong.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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