FNTB Recombinant Monoclonal Antibody
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中文名稱(chēng)：FNTB重組抗體
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貨號(hào)：CSB-RA267691A0HU
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規(guī)格：￥1320
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圖片：
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Western Blot
Positive WB detected in: 293 whole cell lysate, HepG2 whole cell lysate, Hela whole cell lysate, K562 whole cell lysate, Rat brain tissue
All lanes: FNTB antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 49, 44 kDa
Observed band size: 49 kDa -
IHC image of CSB-RA267691A0HU diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. -
Immunoprecipitating FNTB in K562 whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-RA267691A0HU in K562 whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-RA267691A0HU(2μg)+ K562 whole cell lysate(500μg)
Lane 3: K562 whole cell lysate (10μg)
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：CSB-RA267691A0HU FNTB重組單克隆抗體是針對(duì)法尼基轉(zhuǎn)移酶β亞基（FNTB）研發(fā)的高特異性檢測(cè)工具。FNTB作為法尼基轉(zhuǎn)移酶復(fù)合體的關(guān)鍵催化亞基，通過(guò)介導(dǎo)Ras等蛋白的法尼基化修飾參與細(xì)胞增殖與信號(hào)轉(zhuǎn)導(dǎo)調(diào)控，其異常表達(dá)與多種腫瘤發(fā)生發(fā)展密切相關(guān)。本產(chǎn)品經(jīng)多種平臺(tái)嚴(yán)格驗(yàn)證：在Western Blot中可清晰識(shí)別天然及重組FNTB蛋白，呈現(xiàn)單一特異性條帶；免疫組化實(shí)驗(yàn)顯示其在組織切片中具有精準(zhǔn)的亞細(xì)胞定位能力；免疫共沉淀驗(yàn)證證實(shí)其能高效富集內(nèi)源性FNTB及其互作蛋白。推薦使用稀釋度覆蓋WB（1:500-5000）、IHC（1:50-200）及IP（1:200-1000）等多種實(shí)驗(yàn)體系，適用于癌癥分子機(jī)制研究、蛋白質(zhì)翻譯后修飾分析以及靶向法尼基化通路藥物的體外篩選等科研場(chǎng)景。該抗體具有批次穩(wěn)定性高、交叉反應(yīng)性低的特點(diǎn)，為探索FNTB在細(xì)胞信號(hào)網(wǎng)絡(luò)中的功能及其病理學(xué)意義提供了可靠工具。
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Uniprot No.：
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基因名：FNTB
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別名：Protein farnesyltransferase subunit beta (FTase-beta) (EC 2.5.1.58) (CAAX farnesyltransferase subunit beta) (Ras proteins prenyltransferase subunit beta), FNTB
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反應(yīng)種屬：Human, Rat
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免疫原：A synthesized peptide derived from human FNTB
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免疫原種屬：Homo sapiens (Human)
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標(biāo)記方式：Non-conjugated
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克隆類(lèi)型：Monoclonal
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抗體亞型：Rabbit IgG
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純化方式：Affinity-chromatography
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克隆號(hào)：5H4
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
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產(chǎn)品提供形式：Liquid
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應(yīng)用范圍：ELISA, WB, IHC, IP
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推薦稀釋比：
Application Recommended Dilution WB 1:500-1:5000 IHC 1:50-1:200 IP 1:200-1:1000 -
Protocols：
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儲(chǔ)存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能：Essential subunit of the farnesyltransferase complex. Catalyzes the transfer of a farnesyl moiety from farnesyl diphosphate to a cysteine at the fourth position from the C-terminus of several proteins having the C-terminal sequence Cys-aliphatic-aliphatic-X.
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蛋白家族：Protein prenyltransferase subunit beta family
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數(shù)據(jù)庫(kù)鏈接：
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