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  4. USO1 Recombinant Monoclonal Antibody

USO1 Recombinant Monoclonal Antibody

  • 中文名稱：
    USO1 Recombinant Monoclonal Antibody
  • 貨號：
    CSB-RA990355A0HU
  • 規格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in: A549 whole cell lysate(30μg), HEK293 whole cell lysate(30μg), K562 whole cell lysate(30μg), U-87MG whole cell lysate(30μg), A431 whole cell lysate(30μg), Mouse brain tissue lysate(30μg), Rat brain tissue lysate(30μg)
      All lanes: USO1 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/40000 dilution
      Predicted band size: 108 kDa
      Observed band size: 108 kDa
      Exposure time：2min
    • IHC image of CSB-RA990355A0HU diluted at 1:100 and staining in paraffin-embedded human gastric cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • Overlay Peak curve showing K562 cells stained with CSB-RA990355A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 其他：

產品詳情

  • Uniprot No.：
  • 基因名：
    USO1
  • 別名：
    General vesicular transport factor antibody; General vesicular transport factor p115 antibody; P115 antibody; Protein USO1 homolog antibody; TAP antibody; Transcytosis associated protein antibody; Transcytosis-associated protein antibody; Uso1 antibody; USO1_HUMAN antibody; VDP antibody; Vesicle docking protein antibody; Vesicle docking protein p115 antibody; Vesicle-docking protein antibody
  • 反應種屬：
    Human, Mouse, Rat
  • 免疫原：
    A synthesized peptide from human USO1 protein
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    13A9
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, IHC, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:50-1:200
    FC 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    General vesicular transport factor required for intercisternal transport in the Golgi stack; it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane. May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity.
  • 基因功能參考文獻：
    1. USO1 gene may be a promising target for the therapy of human multiple myeloma and its diagnosis marker. PMID: 26898451
    2. USO1 gene has a critical role in human colon cancer: USO1 is involved in cell proliferation and cell migration of colon cancer cells. PMID: 26184508
    3. P115 promotes proliferation of gastric cancer cells through an interaction with MIF. PMID: 24379579
    4. Scyl1 interacts with 58K/formiminotransferase cyclodeaminase (FTCD) and golgin p115, and is required for the maintenance of Golgi morphology. PMID: 20209057
    5. p115 plays a critical role in mitosis progression, implicating it as the only known golgin to regulate both mitosis and apoptosis PMID: 21536679
    6. a conserved tethering function of the Golgi protein p115 CTF which promotes p53-ERK interaction for the amplification of the apoptotic signal. PMID: 21147777
    7. Data show that the number of tandem ARM repeats in an ARM fold ranges from 6 to 12. PMID: 20126549
    8. Data show that p115 degradation plays a key role in amplifying the apoptotic response independently of Golgi fragmentation. PMID: 12438416
    9. p115 acts directly, rather than via a tether, to catalyze trans-SNARE complex formation preceding membrane fusion. PMID: 14736916
    10. The results suggest that p115 is involved in vesicular transport between endoplasmic reticulum and the Golgi, along with microtubule networks. PMID: 16256943
    11. The interaction between p115 and Cog2 was found to be essential for Golgi ribbon reformation after the disruption of the ribbon by p115 KD or brefeldin A treatment and recovery by re-expression of p115 or drug wash out, respectively. PMID: 17274799
    12. Golgi organization depends on mutually interacting domains in betaCOP and p115, suggesting that vesicle tethering at the Golgi involves p115 binding to the COPI coat PMID: 18434597
    13. nuclear import of the p115 CTF is required for it to stimulate the apoptotic response and suggest that its mode of action is confined to the nucleus. PMID: 19028683
    14. terminal repeat folds into the armadillo superhelical groove and allows homodimeric association with important implications for p115 mediated multiple protein interactions and tethering PMID: 19247479
    15. p115 is a critical intermediary component in the regulated secretion of MIF from monocytes/macrophages PMID: 19454686

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  • 亞細胞定位：
    Cytoplasm, cytosol. Golgi apparatus membrane; Peripheral membrane protein.
  • 蛋白家族：
    VDP/USO1/EDE1 family
  • 數據庫鏈接：

    HGNC: 30904

    OMIM: 603344

    KEGG: hsa:8615

    UniGene: Hs.744877