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  4. PYGO2 Recombinant Monoclonal Antibody

PYGO2 Recombinant Monoclonal Antibody

  • 中文名稱：
    PYGO2 Recombinant Monoclonal Antibody
  • 貨號：
    CSB-RA989447A0HU
  • 規格：
    ￥1320
  • 圖片：
    • IHC image of CSB-RA989447A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue上腺 performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • Overlay Peak curve showing SH-SY5Y cells stained with CSB-RA989447A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 其他：

產品詳情

  • Uniprot No.：
  • 基因名：
    PYGO2
  • 別名：
    1190004M21Rik antibody; FLJ33226 antibody; PP7910 antibody; PYGO2 antibody; Pygo2 protein antibody; PYGO2_HUMAN antibody; Pygopus 2 antibody; pygopus homolog 2 (Drosophila) antibody; Pygopus homolog 2 antibody
  • 反應種屬：
    Human
  • 免疫原：
    A synthesized peptide from human PYGO2 protein
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    10D11
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, IHC, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    IHC 1:50-1:200
    FC 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    Involved in signal transduction through the Wnt pathway.
  • 基因功能參考文獻：
    1. Elevated PYGO2 expression in primary prostate adenocarcinoma is a potential risk factor for biochemical recurrence. PMID: 28924059
    2. Overexpression of Pygo2 facilitated the expression of P-glycoprotein, which acts as a drug efflux pump, by promoting the transcription of MDR1 at the MDR1 promoter loci, resulting in acceleration of the efflux of paclitaxel in human glioma cells. PMID: 28427190
    3. We also determined the effect of Pygo2 on the sensitivity of breast tumors resistant to doxorubicin in a mouse model. Finally, RNA samples from 64 paired patient tumors (before and after chemotherapy) highly and significantly overexpressed Pygo2 and/or MDR1 after treatment, thus underlining a pivotal role for the Pygo2-mediated Wnt/b-catenin pathway in the clinical chemoresistance of breast cancer. PMID: 26876203
    4. Findings are consistent with a model in which acetylation of Pygo2 by CBP/p300 family members in the active TCF/beta-catenin complex occurs coincident with histone acetylation and may be required for the recycling of Pygo2 away from the complex subsequent to target gene activation. PMID: 27647933
    5. The activation of its expression by ERalpha and/or specificity protein-1 (SP1) suggests hPYGO2 as a theranostic target for hormone therapy responsive and refractory breast cancer. PMID: 26645832
    6. Pygo2 functions as a prognostic factor for glioma due to its up-regulation of H3K4me3 and promotion of MLL1/MLL2 complex recruitment. PMID: 26902498
    7. this study demonstrated that SNPs in the coding region of Pygo2 might be one of the causative factors in idiopathic oligospermia and azoospermia, resulting in male infertility. PMID: 26345837
    8. Our findings suggest that Pygopus-2 may be an important predictor of poor outcome in HCC patients, and could serve as a novel biomarker for HCC. PMID: 25545771
    9. Pygopus-2 over-expression is associated with hepatic carcinoma. PMID: 25871475
    10. Pygo2 is a common node downstream of oncogenic Wnt and Akt signaling pathways. PMID: 26170450
    11. The results of the present study suggest a novel involvement of Pygo in the promotion of rRNA transcription in cancer cells PMID: 23517060
    12. We conclude that abnormal Pygo2 protein expression may be a marker for advanced non-small cell lung cancer PMID: 23865714
    13. PYGO2 is identified as a new molecular marker of invasive tumors in esophageal squamous cell carcinoma. PMID: 23456637
    14. SNPs in the coding region of Pygo2 gene may be one of the causative factors in idiopathic oligospermia and azoospermia, resulting in male infertility. PMID: 23732668
    15. Pygo2 directly occupies the promoters of multiple histone genes and enhances the acetylation of lysine 56 in histone H3. PMID: 22186018
    16. Pygo2 is highly expressed in and promotes the growth of glioma cells PMID: 20361361
    17. The study demonstrated that Pygo2 was highly expressed in glioma tissue and required for growth of glioblastoma cells. PMID: 20204459
    18. Data show that Pygo2 associates with MLL2 histone methyltransferase and STAGA histone acetyltransferase to facilitate their interaction with beta-catenin and Wnt1-induced, TCF/LEF-dependent transactivation in breast cancer cells. PMID: 20937768
    19. Pygo2 PHD is the only known PHD finger that is capable of interacting simultaneously with two functional ligands, B9L and BCL9 PMID: 20637214
    20. The Expression of pygopus 2 protein mRNA levels were significantly higher in the epithelial ovarian cancer cell lines. PMID: 16609037
    21. hPygo2 is highly expressed in, and required for the growth of breast carcinoma cells PMID: 17203217
    22. These results provide new evidence that Elf-1 is involved in transcriptional activation of hPygo2. PMID: 18314487
    23. Data support a model in which the NHD region of Pygopus is required to augment transcriptional activation by a mechanism that includes both transcriptional activation and histone acetylation resulting from the recruitment of the CBP. PMID: 19555349

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  • 亞細胞定位：
    Nucleus.
  • 數據庫鏈接：

    HGNC: 30257

    OMIM: 606903

    KEGG: hsa:90780

    STRING: 9606.ENSP00000357442

    UniGene: Hs.533597