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  4. KDM1B Recombinant Monoclonal Antibody

KDM1B Recombinant Monoclonal Antibody

  • 中文名稱：
    KDM1B Recombinant Monoclonal Antibody
  • 貨號：
    CSB-RA586625A0HU
  • 規格：
    ￥1320
  • 圖片：
    • Flow cytometric analysis of lysine demethylase 1B expression in HepG2 cells using lysine demethylase 1B antibody. Green, isotype control; red, lysine demethylase 1B.
    • Immunocytochemical staining of HepG2 cells with lysine demethylase 1B antibody. Nuclei were stained blue with DAPI; Lysine demethylase 1B was stained magenta with Alexa Fluor? 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
    • Western blotting analysis using lysine demethylase 1B antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with lysine demethylase 1B antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • 其他：

產品詳情

  • Uniprot No.：
  • 基因名：
    KDM1B
  • 別名：
    KDM1B; Lysine Demethylase 1B; LSD2; AOF1; Flavin-Containing Amine Oxidase Domain-Containing Protein 1; Lysine-Specific Histone Demethylase 1B; Lysine-Specific Histone Demethylase; Lysine (K)-Specific Demethylase 1B; DJ298J15.2; BA204B7.3; FLJ34109; FLJ33898; FLJ43328; C6orf193; Amine Oxidase (Flavin Containing) Domain 1; Chromosome 6 Open Reading Frame 193; Amine Oxidase, Flavin Containing 1; EC 1.14.99.66; C6ORF193
  • 反應種屬：
    Human, Mouse, Rat
  • 免疫原：
    Recombinant Human KDM1B protein
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    4C5
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, FC, ICC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:1000-1:5000
    FC 1:200-1:2000
    ICC 1:100-1:1000
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    Histone demethylase that demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. Required for de novo DNA methylation of a subset of imprinted genes during oogenesis. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Demethylates both mono- and di-methylated 'Lys-4' of histone H3. Has no effect on tri-methylated 'Lys-4', mono-, di- or tri-methylated 'Lys-9', mono-, di- or tri-methylated 'Lys-27', mono-, di- or tri-methylated 'Lys-36' of histone H3, or on mono-, di- or tri-methylated 'Lys-20' of histone H4.
  • 基因功能參考文獻：
    1. These results suggest that LSD2 achieves a promoting effect on small cell lung cancer by indirectly regulating TFPI2 expression through the mediation of DNMT3B expression or through the regulation of the demethylation of H3K4me1 in the promoter region of the TFPI2 gene. PMID: 29845195
    2. This review focuses on published small-molecule inhibitors targeted at the two flavin adenine dinucleotide-dependent lysine demethylases, lysine-specific demethylases 1 and 2, and how the inhibitors interact with the tertiary structures of the enzymes. PMID: 28277979
    3. These results demonstrate an important role for LSD2 in regulation of DNA methylation and gene silencing in breast cancer. PMID: 24924415
    4. Histone demethylase LSD2 acts as an E3 ubiquitin ligase and inhibits cancer cell growth through promoting proteasomal degradation of OGT. PMID: 25773598
    5. Using transcriptome and chromatin immunoprecipitation-sequencing analyses, the study revealed that LSD2 represses the genes involved in lipid influx and metabolism through demethylation of histone H3K4. PMID: 25624347
    6. regulation of tissue factor pathway inhibitor-2 (TFPI-2) expression by lysine-specific demethylase 1 and 2 PMID: 25036127
    7. The zinc finger-SWIRM-oxidase domains is required for KDM1B demethylase activity and the binding of FAD. PMID: 23266887
    8. Human LSD2/KDM1b/AOF1 regulates gene transcription by modulating intragenic H3K4me2 methylation. PMID: 20670891

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  • 亞細胞定位：
    Nucleus.
  • 蛋白家族：
    Flavin monoamine oxidase family
  • 數據庫鏈接：

    HGNC: 21577

    OMIM: 613081

    KEGG: hsa:221656

    STRING: 9606.ENSP00000297792

    UniGene: Hs.709336