Western blot All lanes: Bromodomain-containing protein 9 antibody at 2μg/ml + Mouse liver tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 68, 23, 54, 27, 56, 61 kDa Observed band size: 68 kDa
Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA888000ESR2HU at dilution of 1:100
Immunofluorescent analysis of HepG2 cells using CSB-PA888000ESR2HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
A partial recombinant protein of the human BRD9 protein, corresponding to the 338-597 amino acids, was used to immunize the rabbit to synthesize the BRD9 antibody. The resulting BRD9 antibody is an unconjugated IgG isoform that has undergone antigen affinity purification.
To ensure its reliability, this BRD9 polyclonal antibody has been rigorously quality controlled in multiple applications, including ELISA, WB, IHC, and IF. It has been shown to be reactive with human and mouse BRD9 proteins. The BRD9 protein is a subunit of the BAF chromatin remodeling complex, which plays a crucial role in regulating gene expression and chromatin structure. Additionally, BRD9 has been implicated in other cellular processes, such as cell division, DNA damage repair, and cell differentiation.
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貨期:
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用途:
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Plays a role in chromatin remodeling and regulation of transcription. Acts as a chromatin reader that recognizes and binds acylated histones: binds histones that are acetylated and/or butyrylated. Component of SWI/SNF chromatin remodeling subcomplex GBAF that carries out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Orchestrates also the RAD51-RAD54 complex formation and thereby plays a role in homologous recombination (HR).
基因功能參考文獻:
The bromodomains of BRD9, CECR2, and the second bromodomain of TAF1 recognize the longer butyryl mark on histones. In addition, the TAF1 second bromodomain is capable of binding crotonyl marks. PMID: 26365797