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GALE Monoclonal Antibody

  • 中文名稱：
    GALE Monoclonal Antibody
  • 貨號：
    CSB-MA137194
  • 規格：
    ￥1320
  • 圖片：
    • Flow cytometric analysis of GALE expression in HepG2 cells using GALE antibody. Green, isotype control; red, GALE.
    • Immunocytochemical staining of HepG2 cells with GALE antibody. Nuclei were stained blue with DAPI; GALE was stained magenta with Alexa Fluor? 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
    • Western blotting analysis using GALE antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with GALE antibody and HRP-conjugated goat mouse secondary antibody respectively.
  • 其他：

產品詳情

  • 產品名稱：
    Mouse anti-Homo sapiens (Human) GALE Monoclonal antibody
  • Uniprot No.：
  • 基因名：
    GALE
  • 別名：
    GALE; UDP-Galactose-4-Epimerase; UDP-Glucose 4-Epimerase; SDR1E1; Short Chain Dehydrogenase/Reductase Family 1E, Member 1; UDP-N-Acetylgalactosamine 4-Epimerase; UDP-N-Acetylglucosamine 4-Epimerase; Galactose-4-Epimerase, UDP-; UDP-GalNAc 4-Epimerase; UDP-GlcNAc 4-Epimerase; Galactowaldenase; EC 5.1.3.2; Epididymis Secretory Sperm Binding Protein; UDP Galactose-4'-Epimerase; UDP-Galactose 4-Epimerase; EC 5.1.3.7; EC 5.1.3?47; THC13
  • 宿主：
    Mouse
  • 反應種屬：
    Human, Mouse, Rat
  • 免疫原：
    Recombinant Human GALE protein
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Mouse IgG1
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    8H9
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, FC, ICC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:2500
    FC 1:200-1:2000
    ICC 1:100-1:1000
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    Catalyzes two distinct but analogous reactions: the reversible epimerization of UDP-glucose to UDP-galactose and the reversible epimerization of UDP-N-acetylglucosamine to UDP-N-acetylgalactosamine. The reaction with UDP-Gal plays a critical role in the Leloir pathway of galactose catabolism in which galactose is converted to the glycolytic intermediate glucose 6-phosphate. It contributes to the catabolism of dietary galactose and enables the endogenous biosynthesis of both UDP-Gal and UDP-GalNAc when exogenous sources are limited. Both UDP-sugar interconversions are important in the synthesis of glycoproteins and glycolipids.
  • 基因功能參考文獻：
    1. Mutation in UDP-galactose-4'-epimerase gene is associated with UDP-galactose-4'-epimerase deficiency. PMID: 26565537
    2. Data show the protein structure of GALE and its substrate binding and specificity. It is mutated in type III galactosemia. [review] PMID: 26162744
    3. human UDP-galactose 4'-epimerase stability is increased by variants associated with type III galactosemia but decreased by substrate and cofactor binding PMID: 25150110
    4. These data indicated a critical role of GALE in maintaining cartilage homeostasis, and suggested that GALE inhibition might contribute to OA progress. PMID: 25201731
    5. GALE variants can be arranged into three groups depending on the severity of enzyme impairment. PMID: 23644136
    6. P.K161N-hGALE causes its effects by abolishing an important interaction between the protein and the cofactor. PMID: 22613355
    7. study of hGALE crystal structure and demonstration that residue 307 acts as a gatekeeper mediating substrate access to the hGALE active site PMID: 15175331
    8. Resulst describe the relationship among UDP-galactose 4'-epimerase activity, substrate specificity, metabolic balance, and galactose sensitivity in mammalian cells. PMID: 15701638
    9. Data suggest that reduced catalytic efficiency and increased proteolytic susceptibility of UDP-galactose 4-epimerase are causative factors in type III galactosemia. PMID: 16302980
    10. Subtle biochemical and metabolic abnormalities detected in patients expressing these GALE alleles likely reflect, at least in part, the reduced enzymatic activity of the encoded GALE proteins. PMID: 18188677
    11. Our observations show that altered protein stability is due to misfolding and that loss or reduction of enzyme activity is responsible for the molecular defects underlying GALE-deficiency galactosemia. PMID: 19250319
    12. Disease-causing mutations result in a variety of changes to the steady-state parameters. Mostly these are changes in turnover number, kcat. The ability to dimerize is not affected, but some mutants have increased sensitivity to protease digestion. PMID: 16302980

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  • 相關疾病：
    Epimerase-deficiency galactosemia (EDG)
  • 蛋白家族：
    NAD(P)-dependent epimerase/dehydratase family
  • 數據庫鏈接：

    HGNC: 4116

    OMIM: 230350

    KEGG: hsa:2582

    STRING: 9606.ENSP00000363621

    UniGene: Hs.632380