Rat very low density lipoprotein(VLDL)ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (2) 說(shuō)明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

大鼠極低密度脂蛋白(VLDL)酶聯(lián)免疫試劑盒（CSB-E17088r）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的VLDL含量。極低密度脂蛋白（VLDL）是一種運(yùn)輸內(nèi)源性甘油三酯的脂蛋白。其代謝異常與多種疾病相關(guān)，如動(dòng)脈粥樣硬化。研究機(jī)制主要聚焦于其合成、分泌、代謝途徑，以及和相關(guān)受體、酶的相互作用，以探索調(diào)控VLDL水平防治疾病的新策略。試劑盒檢測(cè)范圍為3.12 ng/mL-200 ng/mL，廣泛應(yīng)用于肥胖、糖尿病、動(dòng)脈粥樣硬化等代謝性疾病的基礎(chǔ)研究，也可用于評(píng)估藥物干預(yù)或基因調(diào)控對(duì)脂蛋白代謝的影響，為探索相關(guān)疾病的分子機(jī)制及潛在治療靶點(diǎn)提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說(shuō)明書。
別名：

N/A
縮寫：

VLDL
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

3.12 ng/mL-200 ng/mL
靈敏度：

0.78 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Cardiovascular
測(cè)定原理：

quantitative
測(cè)定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat VLDL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	99
	Range %	91-103
1:200	Average %	87
	Range %	81-92
1:400	Average %	104
	Range %	98-107
1:800	Average %	101
	Range %	92-105

回收率：

The recovery of rat VLDL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	86-99
EDTA plasma (n=4)	98	91-102

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.