Mouse very low density lipoprotein(VLDL)ELISA Kit

產品詳情

文獻引用 (4) 評價 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

小鼠極低密度脂蛋白(VLDL)酶聯(lián)免疫試劑盒（CSB-E17089m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的VLDL含量。VLDL即極低密度脂蛋白，在人體脂質代謝中發(fā)揮重要作用。它由肝臟合成并分泌，能運輸內源性甘油三酯至外周組織。研究發(fā)現(xiàn)，其代謝異常與動脈粥樣硬化等心血管疾病密切相關，深入探究VLDL代謝機制對相關疾病防治意義重大。試劑盒檢測范圍為0.78 ng/mL-50 ng/mL，適用于代謝機制研究、藥物干預效果評估或營養(yǎng)學模型中脂質代謝動態(tài)監(jiān)測，為心血管疾病或代謝綜合征基礎研究提供高效工具。本品僅用于科研，不用于臨床診斷，產品具體參數(shù)及操作步驟詳見產品說明書。
別名：

N/A
縮寫：

VLDL
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.78 ng/mL-50 ng/mL
靈敏度：

0.195 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse VLDL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	92
	Range %	86-98
1:200	Average %	99
	Range %	92-106
1:400	Average %	83
	Range %	80-85
1:800	Average %	89
	Range %	83-97

回收率：

The recovery of mouse VLDL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-97
EDTA plasma (n=4)	95	91-98

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

平均分:

5.0分 - 1 個評價

樣品類型：組織勻漿液肝臟

樣品信息：小鼠

稀釋比：其他 1：5000

產品評價：我用 CSB-E17089m檢測正常和基因敲除小鼠肝臟VLDL含量，其OD值如圖片所示。預期結果和最終檢測結果類似，較為準確。

By 唐老師