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Rat periostin/osteoblast specific factor 2 (POSTN) ELISA kit

  • 中文名稱:
    大鼠骨膜蛋白/成骨細胞特異性因子2(POSTN)酶聯免疫試劑盒
  • 貨號:
    CSB-EL018381RA
  • 規格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 促銷:
  • 其他:

產品詳情

  • 產品描述:
    大鼠骨膜蛋白/成骨細胞特異性因子2(POSTN)酶聯免疫試劑盒(CSB-EL018381RA)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的POSTN含量。POSTN是一個靶點。它與多種生理和病理過程相關,在腫瘤發生、發展中可能起重要作用。研究機制上,關注其在細胞外基質中的功能,以及與腫瘤細胞增殖、遷移、侵襲等的關聯,有望為腫瘤等疾病的治療提供新方向。試劑盒檢測范圍為31.25 ng/mL-2000 ng/mL,適用于骨骼生物學研究、骨損傷修復機制探索、纖維化疾病模型建立及腫瘤微環境相關研究等科研領域,為體外定量分析POSTN在生理或病理條件下的動態變化提供可靠工具。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 縮寫:
  • 種屬:
    Rattus norvegicus (Rat)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    31.25 ng/mL-2000 ng/mL
  • 靈敏度:
    7.81 ng/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Cardiovascular
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rat POSTN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %92
    Range %86-96
    1:2Average %104
    Range %98-108
    1:4Average %90
    Range %85-94
    1:8Average %98
    Range %92-101
  • 回收率:
    The recovery of rat POSTN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9184-95
    EDTA plasma (n=4)9890-103
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    20002.565 2.487 2.526 2.343
    10002.140 2.078 2.109 1.926
    5001.383 1.369 1.376 1.193
    2500.842 0.804 0.823 0.640
    1250.475 0.507 0.491 0.308
    62.50.417 0.403 0.410 0.227
    31.250.280 0.272 0.276 0.093
    00.185 0.181 0.183
  • 數據處理:
  • 貨期:
    3-5 working days

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