Rat oncostatin M (OSM) ELISA kit

文獻(xiàn)引用 (1) 說明書下載

產(chǎn)品詳情

產(chǎn)品描述：

大鼠抑瘤素M(OSM)酶聯(lián)免疫試劑盒（CSB-E07402r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的OSM含量。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，本產(chǎn)品適用于科研領(lǐng)域中對大鼠OSM蛋白表達(dá)的定量分析，可應(yīng)用于炎癥性疾病模型、組織再生機(jī)制研究或免疫調(diào)控相關(guān)實(shí)驗(yàn)，為探索OSM在心血管疾病、纖維化病變等領(lǐng)域的分子機(jī)制提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

Osm ELISA Kit; Oncostatin-M ELISA Kit; OSM ELISA Kit
縮寫：

OSM
Uniprot No.：

Q65Z15
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma
檢測范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat OSM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-95
1:2	Average %	104
	Range %	99-108
1:4	Average %	91
	Range %	85-97
1:8	Average %	95
	Range %	91-99

回收率：

The recovery of rat OSM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	92-102
EDTA plasma (n=4)	89	82-93

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Growth regulator. Inhibits the proliferation of a number of tumor cell lines. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells. Uses only type II OSM receptor (heterodimers composed of OSMR and IL6ST). Involved in the maturation of fetal hepatocytes, thereby promoting liver development and regeneration.
基因功能參考文獻(xiàn)：
1. OSM is a key mediator for inducing differentiation of OC15-5 cells into hepatocytes PMID: 15743783
2. In the immortalized mouse and primary cultured proliferative rat hepatocytes, treatment with OSM markedly increased mRNA and protein of claudin-2 together with formation of developed networks of TJ strands. PMID: 17434483
3. OSM may not only play a role in the regulation of Sertoli cell proliferation and the initiation of spermatogenesis but may also play a role in the regulation of Leydig cell progenitor formation. PMID: 17996055
4. Results indicate that osteosarcoma cells stably producing OSM do not develop resistance to this cytokine and thus could be a valuable new tool to study the anti-cancer effect of OSM in vivo. PMID: 19168167
亞細(xì)胞定位：

Secreted.
蛋白家族：

LIF/OSM family
組織特異性：

Widely expressed. Expressed at higher levels in liver, skin and spleen.
數(shù)據(jù)庫鏈接：

KEGG: rno:289747

STRING: 10116.ENSRNOP00000032548

UniGene: Rn.127158