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  4. Rat leukemia inhibitory factor,LIF ELISA Kit

Rat leukemia inhibitory factor,LIF ELISA Kit

  • 中文名稱：
    大鼠白血病抑制因子(LIF)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E07432r
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3900/￥2500
  • 促銷：
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠白血病抑制因子(LIF)酶聯(lián)免疫試劑盒（CSB-E07432r）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、支氣管肺泡灌洗液、組織勻漿樣本中的LIF含量。LIF（白血病抑制因子）是一種多效性細(xì)胞因子，屬于IL-6家族，參與細(xì)胞生長(zhǎng)、分化和免疫反應(yīng)。其研究機(jī)制涉及調(diào)節(jié)細(xì)胞增殖、分化和表型，影響骨代謝、神經(jīng)發(fā)育等。LIF通過與其受體結(jié)合，激活JAK/STAT和MAPK信號(hào)通路，參與多種疾病的發(fā)病過程。試劑盒檢測(cè)范圍為0.312 ng/mL-20 ng/mL，適用于呼吸系統(tǒng)疾病模型研究、干細(xì)胞分化調(diào)控實(shí)驗(yàn)及炎癥相關(guān)機(jī)制探索，可為肺纖維化、急性肺損傷等動(dòng)物模型中的LIF動(dòng)態(tài)監(jiān)測(cè)提供可靠工具，助力科研人員深入解析LIF在組織再生、免疫微環(huán)境調(diào)節(jié)中的生物學(xué)功能。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    LifLeukemia inhibitory factor ELISA Kit; LIF ELISA Kit; Cholinergic neuronal differentiation factor ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, bronchoalveolar lavage fluid, tissue homogenates
  • 檢測(cè)范圍：
    0.312 ng/mL-20 ng/mL
  • 靈敏度：
    0.078 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Cancer
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat LIF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %89
    Range %84-95
    1:2Average %93
    Range %89-97
    1:4Average %99
    Range %93-104
    1:8Average %90
    Range %86-98
  • 回收率：
    The recovery of rat LIF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8782-92
    EDTA plasma (n=4)9589-99
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    202.249 2.174 2.212 2.039
    101.583 1.561 1.572 1.399
    51.012 0.952 0.982 0.809
    2.50.599 0.583 0.591 0.418
    1.250.446 0.416 0.431 0.258
    0.6250.320 0.299 0.310 0.137
    0.3120.258 0.245 0.252 0.079
    00.177 0.169 0.173 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.
  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    LIF/OSM family
  • 數(shù)據(jù)庫鏈接：


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