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Rabbit Immunoglobulin A,IgA ELISA Kit

  • 中文名稱:
    兔子免疫球蛋白A(IgA)酶聯免疫試劑盒
  • 貨號:
    CSB-E06946Rb
  • 規格:
    96T/48T
  • 價格:
    ¥4200/¥3000
  • 促銷:
  • 其他:

產品詳情

  • 產品描述:
    兔子免疫球蛋白A(IgA)酶聯免疫試劑盒(CSB-E06946Rb)為競爭法ELISA試劑盒,定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的IgA含量。IgA即免疫球蛋白A,是人體黏膜免疫的主要抗體,在抵御病原體入侵、維持黏膜穩態等方面有重要作用。其研究機制主要聚焦于IgA與抗原的識別結合、參與免疫調節過程,以及在黏膜相關疾病如感染性疾病、自身免疫病中的作用機制。試劑盒檢測范圍為12 ng/mL-3000 ng/mL,尤其適用于疫苗研究、腸道菌群互作實驗或慢性炎癥模型中黏膜免疫效能的評估,也可用于體外細胞實驗探究免疫調節因子對IgA分泌的影響。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 縮寫:
    IgA
  • 種屬:
    Oryctolagus cuniculus (Rabbit)
  • 樣本類型:
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍:
    12 ng/mL-3000 ng/mL
  • 靈敏度:
    3 ng/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Immunology
  • 測定原理:
    quantitative
  • 測定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IgA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1000Average %89
    Range %84-97
    1:2000Average %91
    Range %89-96
    1:4000Average %93
    Range %87-104
    1:8000Average %92
    Range %86-98
  • 回收率:
    The recovery of rabbit IgA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9788-103
    EDTA plasma (n=4)9585-107
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2Average
    30000.221 0.218 0.220
    7500.351 0.354 0.353
    187.50.629 0.625 0.627
    471.464 1.502 1.483
    122.259 2.235 2.247
    03.218 3.155 3.187
  • 數據處理:
  • 貨期:
    3-5 working days

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