Rat Immunoglobulin A,IgA ELISA Kit

產品詳情

文獻引用 (5) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

大鼠免疫球蛋白A(IgA)酶聯免疫試劑盒（CSB-E07987r）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的IgA含量。IgA作為靶點有重要意義。IgA是人體黏膜免疫的主要抗體。研究機制上，圍繞其與病原體相互作用、在黏膜表面防御機制等開展，旨在通過干預IgA相關通路，研發治療感染性疾病、自身免疫病等的藥物，為攻克相關病癥提供新策略。試劑盒檢測范圍為7.81 ng/mL-2000 ng/mL，本產品適用于免疫學研究、感染性疾病模型建立、疫苗開發評估等科研領域，特別適用于長期監測實驗動物體液免疫應答動態，或探究免疫調節劑、營養干預等外界因素對大鼠IgA分泌的影響，為免疫相關機制研究提供可靠的數據支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

IgA
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma
檢測范圍：

7.81 ng/mL-2000 ng/mL
靈敏度：

7.81 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat IgA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	101
	Range %	97-104
1:2000	Average %	92
	Range %	88-96
1:4000	Average %	90
	Range %	86-94
1:8000	Average %	103
	Range %	99-107

回收率：

The recovery of rat IgA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	88-98
EDTA plasma (n=4)	93	89-97

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.