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  4. Mouse Macrophage Inflammatory Protein 1β,MIP-1β ELISA kit

Mouse Macrophage Inflammatory Protein 1β,MIP-1β ELISA kit

  • 中文名稱：
    小鼠巨噬細胞炎性蛋白1β(MIP-1β/CCL4)酶聯免疫試劑盒
  • 貨號：
    CSB-E04665m-IS
  • 規格：
    96T/48T
  • 價格：
    ￥3200/￥2500
  • 促銷：
  • 其他：

產品詳情

  • 產品描述：
    小鼠巨噬細胞炎性蛋白1β(MIP-1β/CCL4)酶聯免疫試劑盒（CSB-E04665m-IS）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的CCL4含量。CCL4即趨化因子配體4，它在免疫調節等生理過程有重要作用。其背景與免疫細胞的募集和活化相關。研究機制方面，主要通過與相應受體結合，引導免疫細胞遷移至炎癥或損傷部位，參與免疫反應的調控，在感染、腫瘤等疾病研究中受關注。試劑盒檢測范圍為3.12 pg/mL-200 pg/mL，靈敏度為1.56 pg/mL。適用于體外研究炎癥反應機制、免疫細胞遷移調控、疾病模型構建等科研場景，可通過標準曲線精確分析樣本中MIP - 1β的動態濃度變化，為探索趨化因子在免疫病理過程中的作用提供實驗支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    Ccl4 ELISA Kit; Mip1b ELISA Kit; Scya4 ELISA Kit; C-C motif chemokine 4 ELISA Kit; Immune activation protein 2 ELISA Kit; ACT-2 ELISA Kit; ACT2 ELISA Kit; Macrophage inflammatory protein 1-beta ELISA Kit; MIP-1-beta ELISA Kit; Protein H400 ELISA Kit; SIS-gamma ELISA Kit; Small-inducible cytokine A4 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍：
    3.12 pg/mL-200 pg/mL
  • 靈敏度：
    1.56 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse MIP-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    102

    Range %

    95-108

    1:2

    Average %

    96

    Range %

    91-102

    1:4

    Average %

    91

    Range %

    87-96

    1:8

    Average %

    90

    Range %

    86-96

  • 回收率：

    The recovery of mouse MIP-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    94

    88-98

    EDTA plasma (n=4)

    97

    91-103

  • 標準曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    200

    2.483

    2.634

    2.558

    2.386

    100

    1.530

    1.617

    1.574

    1.401

    50

    1.009

    0.957

    0.983

    0.811

    25

    0.557

    0.529

    0.543

    0.371

    12.5

    0.350

    0.331

    0.340

    0.168

    6.25

    0.244

    0.253

    0.249

    0.076

    3.12

    0.196

    0.184

    0.190

    0.018

    0

    0.177

    0.168

    0.172

    　

  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Monokine with inflammatory and chemokinetic properties.
  • 基因功能參考文獻：
    1. CCL4-CCR5 axis can contribute to breast cancer metastasis to bone by mediating the interaction between cancer cells and fibroblasts in bone cavity. PMID: 27177471
    2. YY1 regulates lung CCL4 transcription in pulmonary tuberculosis. PMID: 26786659
    3. Ccl4 mRNAs increased within 5 h after injury in mouse cortical slices. PMID: 25895671
    4. CCL4 and CCL5 expression was higher in livers of infected WSX-1(-/-) mice than infected WT mice, and hepatic CD4 T cells from WSX-1(-/-) mice expressed higher levels of CCR5 than cells from WT mice PMID: 24244314
    5. CCL4 upregulation was associated with increased Snail expression and downregulation of p53/PTEN in high-grade PIN and prostate cancer PMID: 23878190
    6. These results suggest that MIP-1beta is a novel key mediator, and the peripheral MIP-1beta-CCR5 axis contributes to neuropathic pain. PMID: 22528550
    7. results indicate that MIP-1beta is involved in the recruitment of bone marrow-derived monocyte lineage cells PMID: 21912378
    8. increased responsiveness of murine eosinophils to MIP-1beta is mediated by CCR5 receptor in mice PMID: 12050188
    9. macrophage inflammatory protein-1beta is a hypoxia-induced neutrophil survival factor. PMID: 15630139
    10. MIP-1beta is overexpressed, and VE-cadherin is underexpressed in heart transplant allografts compared with isografts PMID: 15897346
    11. Overproduction of MIP-1beta in chemokine (C-C motif) receptor 5-deficient mice after collagen II-immunization may contribute partially to the occurrence of arthritis PMID: 15967376
    12. The expression and role CXCL12 and CCL4 and their receptors (CXCR4 and CCR5) in regulating thymocyte migration in conjunction with extracellular matrix during acute T. cruzi infection are investigated. PMID: 16637021
    13. ATF3 appears to be part of a control mechanism that limits the amount of CCL4 released by macrophages, preventing excessive inflammation. PMID: 16982098
    14. Antibody neutralization of CCL4 abrogates the ability of T-cells from IL-4-treated NOD mice to transfer protection against type 1 diabetes. PMID: 17327452

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  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Intercrine beta (chemokine CC) family
  • 數據庫鏈接：


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