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Human anti-double stranded DNA(dsDNA) antibody(IgG) ELISA Kit

  • 中文名稱:
    人抗雙鏈DNA(dsDNA)抗體/天然DNA抗體(IgG)酶聯免疫試劑盒
  • 貨號:
    CSB-E04911h
  • 規格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 促銷:
  • 其他:

產品詳情

  • 產品描述:
    人抗雙鏈DNA(dsDNA)抗體/天然DNA抗體(IgG)酶聯免疫試劑盒(CSB-E04911h)為間接法ELISA試劑盒,定性檢測血清樣本中的dsDNA Ab (IgG)含量。dsDNA Ab (IgG)即抗雙鏈 DNA 抗體(IgG 型),其產生與機體自身免疫紊亂有關,常見于系統性紅斑狼瘡等自身免疫病。研究認為它可與體內雙鏈 DNA 結合形成免疫復合物,沉積于組織引發炎癥反應,干擾正常生理功能,是相關疾病重要研究靶點。本產品適用于科研場景中SLE相關抗體水平分析、疾病活動度關聯研究,或用于評估實驗性治療對自身免疫反應的影響,為免疫病理學研究及藥物開發提供可靠工具。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    N/A
  • 縮寫:
    dsDNA Ab (IgG)
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Autoimmunity
  • 測定原理:
    qualitative
  • 測定方法:
    Indirect
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<15%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<15%

    Three samples of known concentration were tested in twenty assays to assess.

  • 標準曲線:

    Test parameter

    specification

    test result

    Positive control

    >0.6

    1.246

    Negative control

    <0.15

    0.053

    Positive rate

    10,Positive

    100%

    Negative rate

    10,Negative

    100%

  • 本試劑盒所含材料:
    • A micro ELISA plate --- The 96-well plate has been pre-coated with antigen-specific for dsDNA Ab (IgG). This dismountable microplate can be divided into 12 x 8 strip plates.
    • Negative Control (1 x 800 μl)
    • Positive Control (1 x 800 μl)
    • HRP-conjugate (1 x10 ml) --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
    • Sample Diluent (1 x 20 ml) --- Dilute the standard and sample solution.
    • Wash Buffer (25 x concentrate) (1 x20 ml) --- Wash away unbound or free substances.
    • Substrate A 1 x 5 ml --- Mix with substrate B, and the TMB is catalyzed by HRP to elicit a chromogenic signal.
    • Substrate B 1 x 5 ml --- Mix with substrate A, and the TMB is catalyzed by HRP to elicit a chromogenic signal.
    • Stop Solution (1 x 5 ml) --- Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
    • An instruction manual

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  • 本試劑盒不含材料:
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
    • An incubator that can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

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  • 貨期:
    3-5 working days

產品評價

平均分:
5.0分 - 1 個評價

樣品類型:血清 ,血漿(抗凝劑) EDTA

樣品信息:小鼠

稀釋比:其他 1:10

產品評價: 我用華美生物貨號為CSB-E04911h的ELISA kit檢測空白或人源化PBMC重建小鼠模型血清、正常人或炎癥患者中的anti-dsDNA的水平,樣本上樣前給予不同的稀釋倍數,再分析OD值,確認cutoff 值和最終的樣本稀釋倍數。

By 張老師



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