Human Protein notum homolog(NOTUM) ELISA kit

產品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

人notum蛋白同源物(NOTUM)酶聯免疫試劑盒（CSB-EL015955HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的NOTUM含量。NOTUM作為一個靶點，它是羧基酯酶家族成員。在研究機制上，其可通過去除Wnt蛋白的棕櫚酰基抑制Wnt信號通路。在組織發育、干細胞維持等生理過程以及腫瘤、神經退行性疾病等病理進程中發揮作用，是潛在治療靶點。試劑盒檢測范圍為1.56 ng/mL-100 ng/mL，在組織再生、脂肪代謝及神經退行性疾病研究中具有重要科研價值。適用于探究NOTUM在代謝性疾病、腫瘤微環境調控或神經發育相關機制中的表達變化，為體外實驗研究提供可靠的蛋白定量支持，滿足基礎科研中對Wnt通路相關分子調控機制的研究需求。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

NOTUM ELISA Kit; notum pectinacetylesterase homolog (Drosophila) ELISA Kit; Notum pectinacetylesterase homolog ELISA Kit; NOTUM_HUMAN ELISA Kit; Protein notum homolog ELISA Kit
縮寫：

NOTUM
Uniprot No.：

Q6P988
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

1.56 ng/mL-100 ng/mL
靈敏度：

0.39 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Neuroscience
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human NOTUM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	90-100
1:2	Average %	89
	Range %	85-93
1:4	Average %	92
	Range %	88-96
1:8	Average %	85
	Range %	80-90

回收率：

The recovery of human NOTUM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-100
EDTA plasma (n=4)	106	100-114

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Carboxylesterase that acts as a key negative regulator of the Wnt signaling pathway by specifically mediating depalmitoleoylation of WNT proteins. Serine palmitoleoylation of WNT proteins is required for efficient binding to frizzled receptors.
基因功能參考文獻：
1. NOTUM expression was increased in metastatic cells. Proliferation was suppressed by inhibiting expression of NOTUM. Knockdown of NOTUM genes inhibited proliferation as well as migration, with possible involvement of p38 and c-JUN N-terminal kinase in this process. PMID: 30343282
2. Data show that notum and glypican-1 and glypican-3 gene expression during colorectal cancer (CRC) development and present evidence to suggest them as potential new biomarkers of CRC pathogenesis. PMID: 26517809
3. Kinetic and mass spectrometric analyses of human proteins show that Notum is a carboxylesterase that removes an essential palmitoleate moiety from Wnt proteins and thus constitutes the first known extracellular protein deacylase PMID: 25731175
4. Overexpression of NOTUM is associated with hepatocellular carcinoma PMID: 18429952
亞細胞定位：

Secreted.
蛋白家族：

Pectinacetylesterase family, Notum subfamily
組織特異性：

Rarely expressed in adult normal tissues.
數據庫鏈接：

HGNC: 27106

OMIM: 609847

KEGG: hsa:147111

STRING: 9606.ENSP00000387310

UniGene: Hs.106137