Fish transforming growth factor, beta 1 (TGFB1) ELISA kit

說明書下載

產(chǎn)品詳情

產(chǎn)品描述：

魚轉(zhuǎn)化生長因子β1(TGFB1)酶聯(lián)免疫試劑盒（CSB-EL023446FI）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿樣本中的TGFB1含量。TGFB1 即轉(zhuǎn)化生長因子 -β1，在細(xì)胞生長、分化、凋亡等多種生物學(xué)進(jìn)程中發(fā)揮關(guān)鍵作用。其異常表達(dá)與腫瘤、纖維化等多種疾病密切相關(guān)。研究機(jī)制主要聚焦于其信號(hào)通路，通過調(diào)節(jié)下游基因表達(dá)影響細(xì)胞行為，為相關(guān)疾病治療提供潛在靶點(diǎn)。試劑盒檢測(cè)范圍為6.25 ng/mL-400 ng/mL，適用于科研領(lǐng)域探究環(huán)境脅迫、病原感染或營養(yǎng)代謝對(duì)魚類免疫系統(tǒng)的影響，也可用于水產(chǎn)養(yǎng)殖中魚類健康狀態(tài)評(píng)估及免疫相關(guān)分子通路研究，為水生動(dòng)物免疫學(xué)、病理學(xué)及生態(tài)毒理學(xué)研究提供可靠的分析工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

TGFB1
種屬：

Fish
反應(yīng)時(shí)間：

1-5h
上樣量：

50-100ul
檢測(cè)波長：

450 nm
研究領(lǐng)域：

Signal Transduction
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of fish TGFB1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	88-95
1:2	Average %	92
	Range %	85-105
1:4	Average %	97
	Range %	90-102
1:8	Average %	95
	Range %	90-105

回收率：

The recovery of fish TGFB1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	82-98
EDTA plasma (n=4)	94	85-99

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.