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Fish adrenocorticotropic hormone (ACTH) ELISA kit

  • 中文名稱:
    魚促腎上腺皮質激素(ACTH)酶聯免疫試劑盒
  • 貨號:
    CSB-E15926Fh
  • 規格:
    96T/48T
  • 價格:
    ¥3800/¥2500
  • 促銷:
  • 其他:

產品詳情

  • 產品描述:
    魚促腎上腺皮質激素(ACTH)酶聯免疫試劑盒(CSB-E15926Fh)為競爭法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的ACTH含量。促腎上腺皮質激素(ACTH)由腺垂體分泌,能促進腎上腺皮質增生及腎上腺皮質激素合成與釋放。其研究機制主要聚焦于調控糖皮質激素分泌、參與應激反應等。深入研究它能助力理解內分泌系統運行,為相關疾病治療提供新思路。試劑盒檢測范圍為75 pg/mL-1200 pg/mL,對研究魚類內分泌調節機制、環境應激適應性及養殖健康管理具有重要意義;支持科研人員開展魚類生理狀態評估、水環境毒理學研究及水產養殖中的激素水平監測;為探究魚類神經內分泌系統功能、繁殖調控及應激病理機制提供可靠工具本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 縮寫:
    ACTH
  • 種屬:
    Fish
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    75 pg/mL-1200 pg/mL
  • 靈敏度:
    75 pg/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Metabolism
  • 測定原理:
    quantitative
  • 測定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of fish ACTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 105  
    Range % 94-109  
    1:2 Average % 92  
    Range % 84-97  
    1:4 Average % 101  
    Range % 98-105  
    1:8 Average % 95  
    Range % 84-98  
  • 回收率:
    The recovery of fish ACTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 92 82-97  
    EDTA plasma (n=4) 94 90-98  
                 
                 
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    1200 0.149 0.152 0.151    
    600 0.300 0.288 0.294    
    300 0.477 0.491 0.484    
    150 0.871 0.889 0.880    
    75 1.835 1.887 1.861    
  • 數據處理:
  • 貨期:
    3-5 working days

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