Txnip
Txnip,全稱為硫氧還蛋白相互作用蛋白,是一種多功能蛋白,主要通過與硫氧還蛋白(Trx)結合來調節細胞的氧化還原狀態。它也被稱為VDUP-1或TBP-2,屬于α-抑制蛋白家族成員。Txnip在多種生理和病理過程中扮演重要角色,包括糖尿病及其并發癥、動脈粥樣硬化、缺血/再灌注損傷和癌癥等。在正常情況下,Txnip位于細胞核內,當細胞內活性氧(ROS)蓄積增多時,會誘導Txnip穿梭到細胞質或線粒體中,通過與Trx結合,抑制Trx的抗氧化能力,有利于ROS的進一步蓄積。當ROS蓄積到毒性濃度時,可誘發內質網應激與線粒體應激,同時激活NLRP3、ASK1等信號蛋白,最終誘導炎癥的產生或細胞凋亡。研究表明,Txnip的表達失衡與多種疾病的發生發展密切相關。例如,在糖尿病患者體內及慢性高血糖環境下,Txnip的表達明顯增加,提示Txnip水平的升高與糖尿病的發生、發展有密切相關。Txnip也可與NLRP3炎性體結合,通過依賴于NLRP3-ASC-caspase-1途徑刺激IL-1β的分泌,誘導炎癥的產生或胰島細胞凋亡。目前,Txnip作為氧化應激效應途徑中的一個關鍵的調控蛋白,已成為評價糖尿病及其并發癥、動脈粥樣硬化、肝細胞缺血/再灌注損傷等疾病的藥物療效的重要靶蛋白。在藥物研發方面,已有針對Txnip的抑制劑(如TIX100、維拉帕米)正在被探索用于治療1型糖尿病。此外,研究提示靶向Txnip或其相關通路(如代謝)可能成為增強ADT敏感性或克服抵抗的新策略。
熱銷產品
Recombinant Thioredoxin-interacting protein (TXNIP) (CSB-CF880966HU)
驗證數據
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
TXNIP Recombinant Monoclonal Antibody (CSB-RA949793A0HU)
驗證數據
Western Blot
Positive WB detected in: THP-1 whole cell lysate(30μg), K562 whole cell lysate(30μg)
All lanes: TXNIP antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 44 kDa kDa
Observed band size: 53 kDa kDa
Exposure time:2min
IHC image of CSB-RA949793A0HU diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Overlay Peak curve showing K562 cells stained with CSB-RA949793A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
TXNIP Antibody (CSB-PA880966LA01HU)
驗證數據
Immunofluorescence staining of MCF-7 cells with CSB-PA880966LA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Human Thioredoxin-interacting protein(TXNIP) ELISA kit (CSB-EL025383HU)
驗證數據
貨號:CSB-EL025383HU
規格:96T/48T
靈敏度:3.9 pg/mL
檢測范圍:15.6 pg/mL-1000 pg/mL
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Txnip Antibodies
Txnip for Mus musculus (Mouse)
| 產品貨號 | 產品名稱 | 種屬反應性 | 應用類型 |
|---|---|---|---|
| CSB-PA803849LA01MO | Txnip Antibody | Mouse | ELISA |
| CSB-PA803849LB01MO | Txnip Antibody, HRP conjugated | Mouse | ELISA |
| CSB-PA803849LC01MO | Txnip Antibody, FITC conjugated | Mouse | |
| CSB-PA803849LD01MO | Txnip Antibody, Biotin conjugated | Mouse | ELISA |
Txnip for Homo sapiens (Human)
| 產品貨號 | 產品名稱 | 種屬反應性 | 應用類型 |
|---|---|---|---|
| CSB-PA880966LA01HU | TXNIP Antibody | Human | ELISA, IF |
| CSB-PA880966LB01HU | TXNIP Antibody, HRP conjugated | Human | ELISA |
| CSB-PA880966LC01HU | TXNIP Antibody, FITC conjugated | Human | |
| CSB-PA880966LD01HU | TXNIP Antibody, Biotin conjugated | Human | ELISA |
| CSB-RA949793A0HU | TXNIP Recombinant Monoclonal Antibody | Human | ELISA, WB, IHC, FC |
Txnip Proteins
Txnip Proteins for Sus scrofa (Pig)
| 產品貨號 | 產品名稱 | 來源 |
|---|---|---|
| CSB-YP639475PI CSB-EP639475PI CSB-BP639475PI CSB-MP639475PI CSB-EP639475PI-B |
Recombinant Pig Thioredoxin-interacting protein (TXNIP) | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
Txnip Proteins for Rattus norvegicus (Rat)
| 產品貨號 | 產品名稱 | 來源 |
|---|---|---|
| CSB-YP683628RA CSB-EP683628RA CSB-BP683628RA CSB-MP683628RA CSB-EP683628RA-B |
Recombinant Rat Thioredoxin-interacting protein (Txnip) | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
Txnip Proteins for Pongo abelii (Sumatran orangutan)
| 產品貨號 | 產品名稱 | 來源 |
|---|---|---|
| CSB-YP732667PYX CSB-EP732667PYX CSB-BP732667PYX CSB-MP732667PYX CSB-EP732667PYX-B |
Recombinant Pongo abelii Thioredoxin-interacting protein (TXNIP) | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
Txnip Proteins for Mus musculus (Mouse)
| 產品貨號 | 產品名稱 | 來源 |
|---|---|---|
| CSB-YP803849MO | Recombinant Mouse Thioredoxin-interacting protein (Txnip) | Yeast |
| CSB-YP808349MO CSB-EP808349MO CSB-BP808349MO CSB-MP808349MO CSB-EP808349MO-B |
Recombinant Mus musculus Thioredoxin-interacting protein | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
| CSB-EP803849MO | Recombinant Mouse Thioredoxin-interacting protein (Txnip) | E.coli |
| CSB-BP803849MO CSB-MP803849MO CSB-EP803849MO-B |
Recombinant Mouse Thioredoxin-interacting protein (Txnip) | Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
Txnip Proteins for Homo sapiens (Human)
| 產品貨號 | 產品名稱 | 來源 |
|---|---|---|
| CSB-YP880966HU CSB-BP880966HU CSB-EP880966HU-B |
Recombinant Human Thioredoxin-interacting protein (TXNIP) | Yeast Baculovirus In Vivo Biotinylation in E.coli |
| CSB-EP880966HU | Recombinant Human Thioredoxin-interacting protein (TXNIP),Escherichia Coli | E.coli |
Txnip Proteins for Homo sapiens(Human)
| 產品貨號 | 產品名稱 | 來源 |
|---|---|---|
| CSB-CF880966HU | Recombinant Thioredoxin-interacting protein (TXNIP) | in vitro E.coli expression system |
| CSB-CF880966HUa6 | Recombinant Thioredoxin-interacting protein (TXNIP) | in vitro E.coli expression system |
Txnip ELISA Kit
Txnip ELISA Kit for Homo sapiens (Human)
| 產品貨號 | 產品名稱 | 樣本類型 | 靈敏度 |
|---|---|---|---|
| CSB-EL025383HU | Human Thioredoxin-interacting protein(TXNIP) ELISA kit | serum, plasma, tissue homogenates | 3.9 pg/mL |
Txnip ELISA Kit for Mus musculus (Mouse)
| 產品貨號 | 產品名稱 | 樣本類型 | 靈敏度 |
|---|---|---|---|
| CSB-EL025383MO | Mouse Thioredoxin-interacting protein(TXNIP) ELISA kit | serum, plasma, tissue homogenates | 0.11 ng/ml |
Txnip ELISA Kit for Rattus norvegicus (Rat)
| 產品貨號 | 產品名稱 | 樣本類型 | 靈敏度 |
|---|---|---|---|
| CSB-EL025383RA | Rat Thioredoxin-interacting protein(TXNIP) ELISA kit | serum, plasma, tissue homogenates, cell lysates | 11.75 pg/mL |






