Western Blot
Positive WB detected in: HepG2 whole cell lysate(30μg), Raji whole cell lysate(30μg), JK whole cell lysate(30μg), LO2 whole cell lysate(30μg), HT29 whole cell lysate(30μg), 293 whole cell lysate(30μg),Mouse Liver tissue lysate(30μg),Rat Liver tissue lysate(30μg)
All lanes: SLC13A5 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 64,59,62 kDa
Observed band size: 64 kDa
Exposure time: 120s

IHC image of CSB-PA768239LA01HU diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody.