(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity
Measured by its binding ability in a functional ELISA.Immobilized Human SIGLEC15 at 5 μg/ml can bind Anti-SIGLEC15 recombinant antibody(CSB-RA761623MA3HU). The EC₅₀ is 12.42-16.08 ng/mL.

The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using CSB-PA975446(SIGLEC15 Antibody) at dilution 1/35, on the right is treated with synthetic peptide. (Original magnification: ×200)

The image on the left is immunohistochemistry of paraffin-embedded Human prostate cancer tissue using CSB-PA975446(SIGLEC15 Antibody) at dilution 1/35, on the right is treated with synthetic peptide. (Original magnification: ×200)el: 12%SDS-PAGE, Lysate: 60 μg, Lane: Mouse bladder tissue, Primary antibody: CSB-PA975446(SIGLEC15 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 seconds