(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity
Measured by its binding ability in a functional ELISA. Immobilized Human PDGFRA at 2 μg/ml can bind Anti-PDGFRA recombinant antibody (CSB-RA017712MA1HU). The EC₅₀ is 0.6497-0.8371 ng/mL.

The purity of PDGFRA was greater than 95% as determined by SEC-HPLC

Western Blot
Positive WB detected in: HEK293 whole cell lysate, Mouse liver tissue
All lanes: PDGFRA antibody at 3μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 123, 25, 83 kDa
Observed band size: 123 kDa

Immunofluorescent analysis of U251 cells using CSB-PA017712LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)