IHC image of CSB-PA016289LA01HU diluted at 1:100 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at 37℃ Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.

Western Blot
Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, SH-SY5Y whole cell lysate, A549 whole cell lysate, HL-60 whole cell lysate
All lanes: OFD1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 117,112,43 KDa
Observed band size: 117 KDa
Exposure time：5min