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NUP98

NUP98,即核孔蛋白98,別名ADAR2、ANUP96、Nup98。NUP98是核孔復合體(NPC)的重要組成部分,主要存在于NPC的胞質區及核質區內。NUP98在NPC的組裝和/或維護中發揮作用,Nup98和Nup96參與雙向跨NPC的運輸。NUP98對RNA從細胞核向細胞質的運輸調控作用,其N末端的FG重復結構域是跨核運輸的大分子在NPC上的錨定位點,而C末端含有RNA的結合序列,參與RNA核漿運輸。NUP98的異常表達或融合基因的形成與多種髓系腫瘤的發生發展相關,其通過染色體易位等方式形成融合基因,在多種信號分子的相互作用下,調控下游轉錄因子表達,從而干擾細胞的有絲分裂、基因轉錄及核漿運輸等過程。

熱銷產品

Recombinant Human Nuclear pore complex protein Nup98-Nup96 (NUP98), partial (CSB-MP016209HU)

驗證數據

CSB-MP016209HU

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

NUP98 Recombinant Monoclonal Antibody (CSB-RA995655A0HU)

驗證數據

CSB-RA995655A0HU

Western Blot
Positive WB detected in: K562 whole cell lysate, Hela whole cell lysate
All lanes: NUP98 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 198, 188, 98, 97, 196, 187 kDa
Observed band size: 100 kDa

CSB-RA995655A0HU

IHC image of CSB-RA995655A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

CSB-RA995655A0HU

Immunofluorescence staining of Hela Cells with CSB-RA995655A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

NUP98 Antibodies

NUP98 for Homo sapiens (Human)

NUP98 Proteins

NUP98 Proteins for Rattus norvegicus (Rat)

NUP98 Proteins for Homo sapiens (Human)

NUP98 Proteins for Dictyostelium discoideum (Slime mold)



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