(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Flow cytometric analysis of methylmalonyl-CoA mutase expression in HepG2 cells using methylmalonyl-CoA mutase antibody. Green, isotype control; red, methylmalonyl-CoA mutase.

Immunocytochemical staining of HepG2 cells with methylmalonyl-CoA mutase antibody. Nuclei were stained blue with DAPI; Methylmalonyl-CoA mutase was stained magenta with Alexa Fluor? 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Very Low. Scale bar, 20 μm.

Western blotting analysis using methylmalonyl-CoA mutase antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with methylmalonyl-CoA mutase antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.