CTCF
CTCF，即CCCTC結(jié)合因子，別名11-zinc finger protein、CTCFL paralog。CTCF是一種多功能轉(zhuǎn)錄因子，含有11個(gè)高度保守的鋅指結(jié)構(gòu)域，能夠與DNA結(jié)合并發(fā)揮功能，如介導(dǎo)募集cohesin、參與X染色體失活、異染色質(zhì)邊界的形成、V(D)J重組、RNA剪切、凋亡等。CTCF通過(guò)識(shí)別特定的DNA序列基序結(jié)合到染色質(zhì)上，并作為錨點(diǎn)固定染色質(zhì)環(huán)，是構(gòu)建染色質(zhì)三維結(jié)構(gòu)的基礎(chǔ)。CTCF在細(xì)胞生長(zhǎng)與增殖、系統(tǒng)發(fā)生以及腫瘤發(fā)生等方面都有著重要的作用。CTCF的缺失會(huì)導(dǎo)致多種細(xì)胞分化和組織發(fā)育缺陷，包括影響記憶和學(xué)習(xí)能力、心血管發(fā)育、血液細(xì)胞分化以及肢端發(fā)育等。CTCF通過(guò)建立細(xì)胞譜系特異染色質(zhì)高級(jí)結(jié)構(gòu)調(diào)控決定細(xì)胞命運(yùn)基因表達(dá)的分子機(jī)制，在多種生物學(xué)程序中發(fā)揮關(guān)鍵作用。
熱銷產(chǎn)品
Recombinant Human Transcriptional repressor CTCF (CTCF), partial (CSB-CF006138HU1)
驗(yàn)證數(shù)據(jù)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
CTCF Antibody (CSB-PA006138DSR2HU)
驗(yàn)證數(shù)據(jù)
Western blot
All lanes: Transcriptional repressor CTCF antibody at 6μg/ml
Lane 1: PC-3 whole cell lysate
Lane 2: MCF-7 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 83, 46 kDa
Observed band size: 83 kDa
Immunohistochemistry of paraffin-embedded human ovarian cancer using CSB-PA006138DSR2HU at dilution of 1:100
Chromatin Immunoprecipitation Hela (1.2*106) were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4μg anti-CTCF or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers (CSB-PP006138HU) against the H19ICR promoter.
CTCF Antibodies
CTCF for Homo sapiens (Human)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱 | 種屬反應(yīng)性 | 應(yīng)用類型 |
|---|---|---|---|
| CSB-PA006138GA01HU | CTCF Antibody | Human,Mouse,Rat | ELISA,WB,IHC |
| CSB-PA006138DSR2HU | CTCF Antibody | Human | ELISA, WB, IHC, ChIP |
| CSB-PA006138DSR1HU | CTCF Antibody | Human | ELISA, IHC |
CTCF Proteins
CTCF Proteins for Homo sapiens (Human)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱 | 來(lái)源 |
|---|---|---|
| CSB-YP006138HU1 CSB-EP006138HU1 CSB-BP006138HU1 CSB-MP006138HU1 CSB-EP006138HU1-B |
Recombinant Human Transcriptional repressor CTCF (CTCF), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
| CSB-CF006138HU1 | Recombinant Human Transcriptional repressor CTCF (CTCF), partial | in vitro E.coli expression system |
CTCF Proteins for Gallus gallus (Chicken)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱 | 來(lái)源 |
|---|---|---|
| CSB-YP600891CH CSB-EP600891CH CSB-BP600891CH CSB-MP600891CH CSB-EP600891CH-B |
Recombinant Chicken Transcriptional repressor CTCF (CTCF), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CTCF Proteins for Mus musculus (Mouse)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱 | 來(lái)源 |
|---|---|---|
| CSB-YP726738MO1 CSB-EP726738MO1 CSB-BP726738MO1 CSB-MP726738MO1 CSB-EP726738MO1-B |
Recombinant Mouse Transcriptional repressor CTCF (Ctcf), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CTCF Proteins for Rattus norvegicus (Rat)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱 | 來(lái)源 |
|---|---|---|
| CSB-YP874175RA CSB-EP874175RA CSB-BP874175RA CSB-MP874175RA CSB-EP874175RA-B |
Recombinant Rat Transcriptional repressor CTCF (Ctcf) | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
