(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

The purity of COL3A1 was greater than 95% as determined by SEC-HPLC

Western Blot
Positive WB detected in: HepG2 whole cell lysate(30μg), COLO-205 whole cell lysate(30μg), A-549 whole cell lysate(30μg), MCF-7 whole cell lysate(30μg), A-431 whole cell lysate(30μg), SH-SY5Y whole cell lysate(30μg), HEK293 whole cell lysate(30μg), Mouse lung tissue lysate(30μg)
All lanes: Collagen III alpha 1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 139 kDa
Observed band size: 150 kDa
Exposure time：1min

Overlay Peak curve showing HepG2 cells stained with CSB-RA907456A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.