CDK4

CDK4，即細(xì)胞周期蛋白依賴性激酶4，別名P15INK4B結(jié)合伴侶、P16INK4A結(jié)合伴侶、CDKN2A/p16INK4a互作伴侶等。它是一種由CDK4基因編碼的絲氨酸/蘇氨酸激酶，屬于細(xì)胞周期調(diào)控蛋白家族成員。CDK4在細(xì)胞周期的G1期向S期轉(zhuǎn)換中發(fā)揮關(guān)鍵作用，主要通過(guò)與周期蛋白D（cyclin D）形成復(fù)合體，磷酸化視網(wǎng)膜母細(xì)胞瘤蛋白（Rb），從而促進(jìn)Rb蛋白的去磷酸化，釋放E2F家族轉(zhuǎn)錄因子，推動(dòng)細(xì)胞周期進(jìn)程。CDK4在生物學(xué)上的意義非常廣泛，它參與調(diào)控細(xì)胞周期、細(xì)胞增殖、分化和衰老等重要生物學(xué)過(guò)程。CDK4的活性受到多種因素的精細(xì)調(diào)控，包括周期蛋白的合成、降解以及CDK抑制因子（CKIs）的抑制作用。CDK4的功能異常與多種疾病有關(guān)，尤其是與腫瘤的發(fā)生發(fā)展密切相關(guān)。例如，在多種腫瘤中觀察到CDK4的過(guò)表達(dá)或Rb蛋白功能喪失，導(dǎo)致細(xì)胞周期失控和腫瘤細(xì)胞的無(wú)限制增殖。因此，CDK4是腫瘤治療中的一個(gè)潛在靶點(diǎn)，針對(duì)CDK4的小分子抑制劑正在被開(kāi)發(fā)用于治療某些類型的癌癥。

熱銷產(chǎn)品

Recombinant Human Cyclin-dependent kinase 4 (CDK4) (CSB-EP005065HU)

驗(yàn)證數(shù)據(jù)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

CDK4 Recombinant Monoclonal Antibody (CSB-RA965615A0HU)

驗(yàn)證數(shù)據(jù)

Western Blot
Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, MCF-7 whole cell lysate, PC-3 whole cell lysate
All lanes: CDK4 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 34, 21 kDa
Observed band size: 34 kDa

Immunoprecipitating CDK4 in Hela whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-RA965615A0HU in Hela whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/1500)
Lane 2:CSB-RA965615A0HU(2μg)+ Hela whole cell lysate(500μg)
Lane 3: Hela whole cell lysate (10μg)

CDK4 Antibody

CDK4 Protein

CDK4 ELISA Kit

CDK4 Antibodies

CDK4 for Homo sapiens (Human)

產(chǎn)品貨號(hào)	產(chǎn)品名稱	種屬反應(yīng)性	應(yīng)用類型
CSB-PA005065GA01HU	CDK4 Antibody	Human,Mouse,Rat	ELISA,WB,IF
CSB-PA11659D0Rb	CDK4 Antibody, Biotin conjugated	Human	ELISA
CSB-PA11659B0Rb	CDK4 Antibody, HRP conjugated	Human	ELISA
CSB-PA11659C0Rb	CDK4 Antibody, FITC conjugated	Human
CSB-PA11659A0Rb	CDK4 Antibody	Human	ELISA, IHC
CSB-PA275368	CDK4 Antibody	Human	ELISA,WB,IHC
CSB-PA005118	CDK4 Antibody	Human,Mouse,Rat	WB, ELISA
CSB-PA597701	CDK4 Antibody	Human,Mouse,Rat	ELISA,WB,IHC
CSB-RA965615A0HU	CDK4 Recombinant Monoclonal Antibody	Human	ELISA, WB, IP
CSB-RA697694A0HU	CDK4 Recombinant Monoclonal Antibody	Human	ELISA, WB

CDK4 Proteins

CDK4 Proteins for Sus scrofa (Pig)

產(chǎn)品貨號(hào)	產(chǎn)品名稱	來(lái)源
CSB-YP005065PI CSB-EP005065PI CSB-BP005065PI CSB-MP005065PI CSB-EP005065PI-B	Recombinant Pig Cyclin-dependent kinase 4 (CDK4)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

CDK4 Proteins for Homo sapiens (Human)

產(chǎn)品貨號(hào)	產(chǎn)品名稱	來(lái)源
CSB-YP005065HU CSB-MP005065HU CSB-EP005065HU-B	Recombinant Human Cyclin-dependent kinase 4 (CDK4)	Yeast Mammalian cell In Vivo Biotinylation in E.coli
CSB-EP005065HU	Recombinant Human Cyclin-dependent kinase 4 (CDK4)	E.coli

CDK4 Proteins for Mus musculus (Mouse)

產(chǎn)品貨號(hào)	產(chǎn)品名稱	來(lái)源
CSB-YP005065MO CSB-EP005065MO CSB-BP005065MO CSB-MP005065MO CSB-EP005065MO-B	Recombinant Mouse Cyclin-dependent kinase 4 (Cdk4)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

CDK4 Proteins for Rattus norvegicus (Rat)

產(chǎn)品貨號(hào)	產(chǎn)品名稱	來(lái)源
CSB-YP005065RA CSB-EP005065RA CSB-BP005065RA CSB-MP005065RA CSB-EP005065RA-B	Recombinant Rat Cyclin-dependent kinase 4 (Cdk4)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

CDK4 Proteins for Xenopus laevis (African clawed frog)

產(chǎn)品貨號(hào)	產(chǎn)品名稱	來(lái)源
CSB-YP005065XBE CSB-EP005065XBE CSB-BP005065XBE CSB-MP005065XBE CSB-EP005065XBE-B	Recombinant Xenopus laevis Cyclin-dependent kinase 4 (cdk4)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

CDK4 Proteins for Bos taurus (Bovine)

產(chǎn)品貨號(hào)	產(chǎn)品名稱	來(lái)源
CSB-YP005065BO CSB-EP005065BO CSB-BP005065BO CSB-MP005065BO CSB-EP005065BO-B	Recombinant Bovine Cyclin-dependent kinase 4 (CDK4)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli