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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Xenopus tropicalis UPF0539 protein C7orf59 homolog CSB-YP433273XBF
CSB-EP433273XBF
CSB-BP433273XBF
CSB-MP433273XBF
CSB-EP433273XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Monosiga brevicollis Coiled-coil domain-containing protein 42 homolog (30998) CSB-YP433274MOD
CSB-EP433274MOD
CSB-BP433274MOD
CSB-MP433274MOD
CSB-EP433274MOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Monosiga brevicollis SURF1-like protein (18583), partial CSB-YP433275MOD1
CSB-EP433275MOD1
CSB-BP433275MOD1
CSB-MP433275MOD1
CSB-EP433275MOD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Monosiga brevicollis Structure-specific endonuclease subunit SLX1 homolog (8836) CSB-YP433276MOD
CSB-EP433276MOD
CSB-BP433276MOD
CSB-MP433276MOD
CSB-EP433276MOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis D-ribose pyranase (rbsD) CSB-YP433277BON
CSB-EP433277BON
CSB-BP433277BON
CSB-MP433277BON
CSB-EP433277BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Methylthioribose-1-phosphate isomerase 2 (mtnA2) CSB-YP433278BON
CSB-EP433278BON
CSB-BP433278BON
CSB-MP433278BON
CSB-EP433278BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Glucosamine-6-phosphate deaminase (nagB) CSB-YP433279BON
CSB-EP433279BON
CSB-BP433279BON
CSB-MP433279BON
CSB-EP433279BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Thiazole synthase (thiG) CSB-YP433280BON
CSB-EP433280BON
CSB-BP433280BON
CSB-MP433280BON
CSB-EP433280BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Potassium-transporting ATPase A chain (kdpA), partial CSB-YP433281BON
CSB-EP433281BON
CSB-BP433281BON
CSB-MP433281BON
CSB-EP433281BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Exodeoxyribonuclease 7 small subunit (xseB) CSB-YP433282BON
CSB-EP433282BON
CSB-BP433282BON
CSB-MP433282BON
CSB-EP433282BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis UPF0754 membrane protein BcerKBAB4_0766 (BcerKBAB4_0766), partial CSB-YP433283BON1
CSB-EP433283BON1
CSB-BP433283BON1
CSB-MP433283BON1
CSB-EP433283BON1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Transcriptional regulator MntR (mntR) CSB-YP433284BON
CSB-EP433284BON
CSB-BP433284BON
CSB-MP433284BON
CSB-EP433284BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Chaperone protein DnaK (dnaK), partial CSB-YP433285BON
CSB-EP433285BON
CSB-BP433285BON
CSB-MP433285BON
CSB-EP433285BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis 30S ribosomal protein S20 (rpsT) CSB-YP433286BON
CSB-EP433286BON
CSB-BP433286BON
CSB-MP433286BON
CSB-EP433286BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis 3'-5' exoribonuclease yhaM (yhaM) CSB-YP433287BON
CSB-EP433287BON
CSB-BP433287BON
CSB-MP433287BON
CSB-EP433287BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Ribose-5-phosphate isomerase A (rpiA) CSB-YP433288BON
CSB-EP433288BON
CSB-BP433288BON
CSB-MP433288BON
CSB-EP433288BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis GTPase obg (obg) CSB-YP433289BON
CSB-EP433289BON
CSB-BP433289BON
CSB-MP433289BON
CSB-EP433289BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis ATP-dependent helicase/nuclease subunit A (addA), partial CSB-YP433290BON
CSB-EP433290BON
CSB-BP433290BON
CSB-MP433290BON
CSB-EP433290BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Probable malate:quinone oxidoreductase (mqo) CSB-YP433291BON
CSB-EP433291BON
CSB-BP433291BON
CSB-MP433291BON
CSB-EP433291BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis MutS2 protein (mutS2), partial CSB-YP433292BON
CSB-EP433292BON
CSB-BP433292BON
CSB-MP433292BON
CSB-EP433292BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://www.jhhuazhong.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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