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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus weihenstephanensis Initiation-control protein yabA (BcerKBAB4_0028) CSB-YP432413BON
CSB-EP432413BON
CSB-BP432413BON
CSB-MP432413BON
CSB-EP432413BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Bifunctional protein GlmU (glmU) CSB-YP432414BON
CSB-EP432414BON
CSB-BP432414BON
CSB-MP432414BON
CSB-EP432414BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis 50S ribosomal protein L1 (rplA) CSB-YP432415BON
CSB-EP432415BON
CSB-BP432415BON
CSB-MP432415BON
CSB-EP432415BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis UPF0061 protein BcerKBAB4_3221 (BcerKBAB4_3221) CSB-YP432416BON
CSB-EP432416BON
CSB-BP432416BON
CSB-MP432416BON
CSB-EP432416BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis 30S ribosomal protein S7 (rpsG) CSB-YP432417BON
CSB-EP432417BON
CSB-BP432417BON
CSB-MP432417BON
CSB-EP432417BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis 30S ribosomal protein S10 (rpsJ) CSB-YP432418BON
CSB-EP432418BON
CSB-BP432418BON
CSB-MP432418BON
CSB-EP432418BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis 50S ribosomal protein L13 (rplM) CSB-YP432419BON
CSB-EP432419BON
CSB-BP432419BON
CSB-MP432419BON
CSB-EP432419BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Aspartate--ammonia ligase (asnA) CSB-YP432420BON
CSB-EP432420BON
CSB-BP432420BON
CSB-MP432420BON
CSB-EP432420BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis SsrA-binding protein (smpB) CSB-YP432421BON
CSB-EP432421BON
CSB-BP432421BON
CSB-MP432421BON
CSB-EP432421BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Phosphoglycerate kinase (pgk) CSB-YP432422BON
CSB-EP432422BON
CSB-BP432422BON
CSB-MP432422BON
CSB-EP432422BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis 60 kDa chaperonin (groL), partial CSB-YP432423BON
CSB-EP432423BON
CSB-BP432423BON
CSB-MP432423BON
CSB-EP432423BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Deoxyribose-phosphate aldolase (deoC) CSB-YP432424BON
CSB-EP432424BON
CSB-BP432424BON
CSB-MP432424BON
CSB-EP432424BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP432425BON
CSB-EP432425BON
CSB-BP432425BON
CSB-MP432425BON
CSB-EP432425BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Methylthioribose-1-phosphate isomerase 1 (mtnA1) CSB-YP432426BON
CSB-EP432426BON
CSB-BP432426BON
CSB-MP432426BON
CSB-EP432426BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB, partial CSB-YP432427BON
CSB-EP432427BON
CSB-BP432427BON
CSB-MP432427BON
CSB-EP432427BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis ATP synthase epsilon chain (atpC) CSB-YP432428BON
CSB-EP432428BON
CSB-BP432428BON
CSB-MP432428BON
CSB-EP432428BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis UPF0374 protein BcerKBAB4_0443 (BcerKBAB4_0443) CSB-YP432429BON
CSB-EP432429BON
CSB-BP432429BON
CSB-MP432429BON
CSB-EP432429BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Methylenetetrahydrofolate--tRNA- (uracil-5-)-methyltransferase TrmFO CSB-YP432430BON
CSB-EP432430BON
CSB-BP432430BON
CSB-MP432430BON
CSB-EP432430BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Orotidine 5'-phosphate decarboxylase (pyrF) CSB-YP432431BON
CSB-EP432431BON
CSB-BP432431BON
CSB-MP432431BON
CSB-EP432431BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis 50S ribosomal protein L9 (rplI) CSB-YP432432BON
CSB-EP432432BON
CSB-BP432432BON
CSB-MP432432BON
CSB-EP432432BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.jhhuazhong.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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