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重組蛋白

產品名稱 貨號 規格
Recombinant Bacillus pumilus Anthranilate phosphoribosyltransferase (trpD) CSB-YP422386BOL
CSB-EP422386BOL
CSB-BP422386BOL
CSB-MP422386BOL
CSB-EP422386BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus GTPase Der (der) CSB-YP422387BOL
CSB-EP422387BOL
CSB-BP422387BOL
CSB-MP422387BOL
CSB-EP422387BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus NADPH dehydrogenase (namA) CSB-YP422388BOL
CSB-EP422388BOL
CSB-BP422388BOL
CSB-MP422388BOL
CSB-EP422388BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus N utilization substance protein B homolog (nusB) CSB-YP422389BOL
CSB-EP422389BOL
CSB-BP422389BOL
CSB-MP422389BOL
CSB-EP422389BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) CSB-YP422390BOL
CSB-EP422390BOL
CSB-BP422390BOL
CSB-MP422390BOL
CSB-EP422390BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus UPF0365 protein BPUM_2271 (BPUM_2271), partial CSB-YP422391BOL1
CSB-EP422391BOL1
CSB-BP422391BOL1
CSB-MP422391BOL1
CSB-EP422391BOL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus Germination protease (gpr) CSB-YP422392BOL
CSB-EP422392BOL
CSB-BP422392BOL
CSB-MP422392BOL
CSB-EP422392BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus UPF0473 protein BPUM_2377 (BPUM_2377) CSB-YP422393BOL
CSB-EP422393BOL
CSB-BP422393BOL
CSB-MP422393BOL
CSB-EP422393BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus UPF0735 ACT domain-containing protein BPUM_2431 (BPUM_2431) CSB-YP422394BOL
CSB-EP422394BOL
CSB-BP422394BOL
CSB-MP422394BOL
CSB-EP422394BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus 2-isopropylmalate synthase 1 (leuA1), partial CSB-YP422395BOL
CSB-EP422395BOL
CSB-BP422395BOL
CSB-MP422395BOL
CSB-EP422395BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus Ribonuclease HIII (rnhC) CSB-YP422396BOL
CSB-EP422396BOL
CSB-BP422396BOL
CSB-MP422396BOL
CSB-EP422396BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus UPF0173 metal-dependent hydrolase BPUM_2573 (BPUM_2573) CSB-YP422397BOL
CSB-EP422397BOL
CSB-BP422397BOL
CSB-MP422397BOL
CSB-EP422397BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP422398BOL
CSB-EP422398BOL
CSB-BP422398BOL
CSB-MP422398BOL
CSB-EP422398BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus 2-succinylbenzoate--CoA ligase (menE) CSB-YP422399BOL
CSB-EP422399BOL
CSB-BP422399BOL
CSB-MP422399BOL
CSB-EP422399BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus UPF0349 protein BPUM_2879 (BPUM_2879) CSB-YP422400BOL
CSB-EP422400BOL
CSB-BP422400BOL
CSB-MP422400BOL
CSB-EP422400BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus ATP phosphoribosyltransferase regulatory subunit (hisZ) CSB-YP422401BOL
CSB-EP422401BOL
CSB-BP422401BOL
CSB-MP422401BOL
CSB-EP422401BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus Flagellar protein FliT (fliT) CSB-YP422402BOL
CSB-EP422402BOL
CSB-BP422402BOL
CSB-MP422402BOL
CSB-EP422402BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus 50S ribosomal protein L33 3 (rpmG3) CSB-YP422403BOL
CSB-EP422403BOL
CSB-BP422403BOL
CSB-MP422403BOL
CSB-EP422403BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus UPF0059 membrane protein BPUM_3339 (BPUM_3339), partial CSB-YP422404BOL1
CSB-EP422404BOL1
CSB-BP422404BOL1
CSB-MP422404BOL1
CSB-EP422404BOL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus Thiamine-phosphate synthase (thiE) CSB-YP422405BOL
CSB-EP422405BOL
CSB-BP422405BOL
CSB-MP422405BOL
CSB-EP422405BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產生大量蛋白質、修改基因序列和制造有用的商業產品。重組蛋白的技術資源在重組蛋白的開發過程中,除了表達系統之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據他們開發重組蛋白的經驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產,在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經過多年的發展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.jhhuazhong.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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