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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Francisella tularensis subsp. holarctica 30S ribosomal protein S8 (rpsH) CSB-YP417793FDV
CSB-EP417793FDV
CSB-BP417793FDV
CSB-MP417793FDV
CSB-EP417793FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica 30S ribosomal protein S4 (rpsD) CSB-YP417794FDV
CSB-EP417794FDV
CSB-BP417794FDV
CSB-MP417794FDV
CSB-EP417794FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica DNA mismatch repair protein MutS (mutS), partial CSB-YP417795FDV
CSB-EP417795FDV
CSB-BP417795FDV
CSB-MP417795FDV
CSB-EP417795FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica Ubiquinone/menaquinone biosynthesis methyltransferase ubiE (ubiE) CSB-YP417796FDV
CSB-EP417796FDV
CSB-BP417796FDV
CSB-MP417796FDV
CSB-EP417796FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica Probable glycine dehydrogenase [decarboxylating] subunit 1 (gcvPA) CSB-YP417797FDV
CSB-EP417797FDV
CSB-BP417797FDV
CSB-MP417797FDV
CSB-EP417797FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica Probable inorganic polyphosphate/ATP-NAD kinase (ppnK) CSB-YP417798FDV
CSB-EP417798FDV
CSB-BP417798FDV
CSB-MP417798FDV
CSB-EP417798FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica Adenylate kinase (adk) CSB-YP417799FDV
CSB-EP417799FDV
CSB-BP417799FDV
CSB-MP417799FDV
CSB-EP417799FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica Probable rRNA maturation factor (FTA_0935) CSB-YP417800FDV
CSB-EP417800FDV
CSB-BP417800FDV
CSB-MP417800FDV
CSB-EP417800FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica Probable transcriptional regulatory protein FTA_0979 (FTA_0979) CSB-YP417801FDV
CSB-EP417801FDV
CSB-BP417801FDV
CSB-MP417801FDV
CSB-EP417801FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica Peptidyl-tRNA hydrolase (pth) CSB-YP417802FDV
CSB-EP417802FDV
CSB-BP417802FDV
CSB-MP417802FDV
CSB-EP417802FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP417803FDV
CSB-EP417803FDV
CSB-BP417803FDV
CSB-MP417803FDV
CSB-EP417803FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica Alanine--tRNA ligase (alaS), partial CSB-YP417804FDV
CSB-EP417804FDV
CSB-BP417804FDV
CSB-MP417804FDV
CSB-EP417804FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP417805FDV
CSB-EP417805FDV
CSB-BP417805FDV
CSB-MP417805FDV
CSB-EP417805FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica ATP-dependent dethiobiotin synthetase BioD (bioD) CSB-YP417806FDV
CSB-EP417806FDV
CSB-BP417806FDV
CSB-MP417806FDV
CSB-EP417806FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica DNA replication and repair protein recF (recF) CSB-YP417807FDV
CSB-EP417807FDV
CSB-BP417807FDV
CSB-MP417807FDV
CSB-EP417807FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica UvrABC system protein C (uvrC), partial CSB-YP417808FDV
CSB-EP417808FDV
CSB-BP417808FDV
CSB-MP417808FDV
CSB-EP417808FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP417809FDV
CSB-EP417809FDV
CSB-BP417809FDV
CSB-MP417809FDV
CSB-EP417809FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP417810FDV
CSB-EP417810FDV
CSB-BP417810FDV
CSB-MP417810FDV
CSB-EP417810FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP417811FDV
CSB-EP417811FDV
CSB-BP417811FDV
CSB-MP417811FDV
CSB-EP417811FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica RNA pyrophosphohydrolase (rppH) CSB-YP417812FDV
CSB-EP417812FDV
CSB-BP417812FDV
CSB-MP417812FDV
CSB-EP417812FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產(chǎn)生大量蛋白質、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產(chǎn)生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.jhhuazhong.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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