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重組蛋白

產品名稱 貨號 規(guī)格
Recombinant Francisella tularensis subsp. holarctica Bifunctional protein FolD (folD) CSB-YP416513FDV
CSB-EP416513FDV
CSB-BP416513FDV
CSB-MP416513FDV
CSB-EP416513FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica 50S ribosomal protein L28 (rpmB) CSB-YP416514FDV
CSB-EP416514FDV
CSB-BP416514FDV
CSB-MP416514FDV
CSB-EP416514FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica DNA-directed RNA polymerase subunit alpha 2 (rpoA2) CSB-YP416515FDV
CSB-EP416515FDV
CSB-BP416515FDV
CSB-MP416515FDV
CSB-EP416515FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica tRNA (Ile)-lysidine synthase CSB-YP416516FDV
CSB-EP416516FDV
CSB-BP416516FDV
CSB-MP416516FDV
CSB-EP416516FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica SsrA-binding protein (smpB) CSB-YP416517FDV
CSB-EP416517FDV
CSB-BP416517FDV
CSB-MP416517FDV
CSB-EP416517FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica Protein hfq (hfq) CSB-YP416518FDV
CSB-EP416518FDV
CSB-BP416518FDV
CSB-MP416518FDV
CSB-EP416518FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica DNA polymerase IV (dinB) CSB-YP416519FDV
CSB-EP416519FDV
CSB-BP416519FDV
CSB-MP416519FDV
CSB-EP416519FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica Octanoyltransferase (lipB) CSB-YP416520FDV
CSB-EP416520FDV
CSB-BP416520FDV
CSB-MP416520FDV
CSB-EP416520FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica Phosphoglycerate kinase (pgk) CSB-YP416521FDV
CSB-EP416521FDV
CSB-BP416521FDV
CSB-MP416521FDV
CSB-EP416521FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica Leucine--tRNA ligase (leuS), partial CSB-YP416522FDV
CSB-EP416522FDV
CSB-BP416522FDV
CSB-MP416522FDV
CSB-EP416522FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica 3-deoxy-manno-octulosonate cytidylyltransferase (kdsB) CSB-YP416523FDV
CSB-EP416523FDV
CSB-BP416523FDV
CSB-MP416523FDV
CSB-EP416523FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica Recombination protein RecR (recR) CSB-YP416524FDV
CSB-EP416524FDV
CSB-BP416524FDV
CSB-MP416524FDV
CSB-EP416524FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica Glutamine amidotransferase subunit pdxT (pdxT) CSB-YP416525FDV
CSB-EP416525FDV
CSB-BP416525FDV
CSB-MP416525FDV
CSB-EP416525FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica Peptide chain release factor 3 (prfC), partial CSB-YP416526FDV
CSB-EP416526FDV
CSB-BP416526FDV
CSB-MP416526FDV
CSB-EP416526FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP416527FDV
CSB-EP416527FDV
CSB-BP416527FDV
CSB-MP416527FDV
CSB-EP416527FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica NADH-quinone oxidoreductase subunit C (nuoC) CSB-YP416528FDV
CSB-EP416528FDV
CSB-BP416528FDV
CSB-MP416528FDV
CSB-EP416528FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica D-alanine--D-alanine ligase (ddl) CSB-YP416529FDV
CSB-EP416529FDV
CSB-BP416529FDV
CSB-MP416529FDV
CSB-EP416529FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii UPF0133 protein Rcas_2292 (Rcas_2292) CSB-YP416530RIP
CSB-EP416530RIP
CSB-BP416530RIP
CSB-MP416530RIP
CSB-EP416530RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii Enolase (eno) CSB-YP416531RIP
CSB-EP416531RIP
CSB-BP416531RIP
CSB-MP416531RIP
CSB-EP416531RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii Pantothenate synthetase (panC) CSB-YP416532RIP
CSB-EP416532RIP
CSB-BP416532RIP
CSB-MP416532RIP
CSB-EP416532RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產生大量蛋白質、修改基因序列和制造有用的商業(yè)產品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產,在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.jhhuazhong.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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