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重組蛋白

產品名稱 貨號 規格
Recombinant Actinobacillus succinogenes Bifunctional protein HldE (hldE) CSB-YP411142AUG
CSB-EP411142AUG
CSB-BP411142AUG
CSB-MP411142AUG
CSB-EP411142AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Exodeoxyribonuclease 7 small subunit (xseB) CSB-YP411143AUG
CSB-EP411143AUG
CSB-BP411143AUG
CSB-MP411143AUG
CSB-EP411143AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Chromosome partition protein mukF (mukF) CSB-YP411144AUG
CSB-EP411144AUG
CSB-BP411144AUG
CSB-MP411144AUG
CSB-EP411144AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes 2-dehydro-3-deoxyphosphooctonate aldolase (kdsA) CSB-YP411145AUG
CSB-EP411145AUG
CSB-BP411145AUG
CSB-MP411145AUG
CSB-EP411145AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Dihydrodipicolinate reductase (dapB) CSB-YP411146AUG
CSB-EP411146AUG
CSB-BP411146AUG
CSB-MP411146AUG
CSB-EP411146AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Probable hydrogenase nickel incorporation protein HypA (hypA) CSB-YP411147AUG
CSB-EP411147AUG
CSB-BP411147AUG
CSB-MP411147AUG
CSB-EP411147AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Siroheme synthase (cysG) CSB-YP411148AUG
CSB-EP411148AUG
CSB-BP411148AUG
CSB-MP411148AUG
CSB-EP411148AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes 3-deoxy-manno-octulosonate cytidylyltransferase (kdsB) CSB-YP411149AUG
CSB-EP411149AUG
CSB-BP411149AUG
CSB-MP411149AUG
CSB-EP411149AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Glycerol-3-phosphate acyltransferase (plsB), partial CSB-YP411150AUG
CSB-EP411150AUG
CSB-BP411150AUG
CSB-MP411150AUG
CSB-EP411150AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes tRNA (cmo5U34)-methyltransferase CSB-YP411151AUG
CSB-EP411151AUG
CSB-BP411151AUG
CSB-MP411151AUG
CSB-EP411151AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Ribonuclease HII (rnhB) CSB-YP411152AUG
CSB-EP411152AUG
CSB-BP411152AUG
CSB-MP411152AUG
CSB-EP411152AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase (lpxC) CSB-YP411153AUG
CSB-EP411153AUG
CSB-BP411153AUG
CSB-MP411153AUG
CSB-EP411153AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP411154AUG
CSB-EP411154AUG
CSB-BP411154AUG
CSB-MP411154AUG
CSB-EP411154AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes UPF0059 membrane protein Asuc_1994 (Asuc_1994), partial CSB-YP411155AUG1
CSB-EP411155AUG1
CSB-BP411155AUG1
CSB-MP411155AUG1
CSB-EP411155AUG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP411156AUG
CSB-EP411156AUG
CSB-BP411156AUG
CSB-MP411156AUG
CSB-EP411156AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP411157AUG
CSB-EP411157AUG
CSB-BP411157AUG
CSB-MP411157AUG
CSB-EP411157AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Enolase (eno) CSB-YP411158AUG
CSB-EP411158AUG
CSB-BP411158AUG
CSB-MP411158AUG
CSB-EP411158AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Cell division protein FtsP (ftsP) CSB-YP411159AUG
CSB-EP411159AUG
CSB-BP411159AUG
CSB-MP411159AUG
CSB-EP411159AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Putative membrane protein insertion efficiency factor (Asuc_2115) CSB-YP411160AUG
CSB-EP411160AUG
CSB-BP411160AUG
CSB-MP411160AUG
CSB-EP411160AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. 50S ribosomal protein L28 (rpmB) CSB-YP411161MNJ
CSB-EP411161MNJ
CSB-BP411161MNJ
CSB-MP411161MNJ
CSB-EP411161MNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產生大量蛋白質、修改基因序列和制造有用的商業產品。重組蛋白的技術資源在重組蛋白的開發過程中,除了表達系統之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據他們開發重組蛋白的經驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產,在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經過多年的發展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.jhhuazhong.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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