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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Nitratiruptor sp. Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP409001NFA
CSB-EP409001NFA
CSB-BP409001NFA
CSB-MP409001NFA
CSB-EP409001NFA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitratiruptor sp. Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP409002NFA
CSB-EP409002NFA
CSB-BP409002NFA
CSB-MP409002NFA
CSB-EP409002NFA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitratiruptor sp. Glycerol-3-phosphate acyltransferase (plsY), partial CSB-YP409003NFA1
CSB-EP409003NFA1
CSB-BP409003NFA1
CSB-MP409003NFA1
CSB-EP409003NFA1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitratiruptor sp. 60 kDa chaperonin (groL), partial CSB-YP409004NFA
CSB-EP409004NFA
CSB-BP409004NFA
CSB-MP409004NFA
CSB-EP409004NFA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitratiruptor sp. Phosphomethylpyrimidine synthase (thiC) CSB-YP409005NFA
CSB-EP409005NFA
CSB-BP409005NFA
CSB-MP409005NFA
CSB-EP409005NFA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitratiruptor sp. Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP409006NFA
CSB-EP409006NFA
CSB-BP409006NFA
CSB-MP409006NFA
CSB-EP409006NFA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitratiruptor sp. Protein translocase subunit SecA (secA), partial CSB-YP409007NFA
CSB-EP409007NFA
CSB-BP409007NFA
CSB-MP409007NFA
CSB-EP409007NFA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitratiruptor sp. Ribonuclease P protein component (rnpA) CSB-YP409008NFA
CSB-EP409008NFA
CSB-BP409008NFA
CSB-MP409008NFA
CSB-EP409008NFA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitratiruptor sp. 50S ribosomal protein L9 (rplI) CSB-YP409009NFA
CSB-EP409009NFA
CSB-BP409009NFA
CSB-MP409009NFA
CSB-EP409009NFA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitratiruptor sp. tRNA (mo5U34)-methyltransferase CSB-YP409010NFA
CSB-EP409010NFA
CSB-BP409010NFA
CSB-MP409010NFA
CSB-EP409010NFA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitratiruptor sp. Cysteine--tRNA ligase (cysS) CSB-YP409011NFA
CSB-EP409011NFA
CSB-BP409011NFA
CSB-MP409011NFA
CSB-EP409011NFA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitratiruptor sp. RNA pyrophosphohydrolase (rppH) CSB-YP409012NFA
CSB-EP409012NFA
CSB-BP409012NFA
CSB-MP409012NFA
CSB-EP409012NFA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitratiruptor sp. Chaperone protein DnaJ (dnaJ) CSB-YP409013NFA
CSB-EP409013NFA
CSB-BP409013NFA
CSB-MP409013NFA
CSB-EP409013NFA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitratiruptor sp. 5'-nucleotidase surE (surE) CSB-YP409014NFA
CSB-EP409014NFA
CSB-BP409014NFA
CSB-MP409014NFA
CSB-EP409014NFA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitratiruptor sp. 2-isopropylmalate synthase (leuA), partial CSB-YP409015NFA
CSB-EP409015NFA
CSB-BP409015NFA
CSB-MP409015NFA
CSB-EP409015NFA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitratiruptor sp. tRNA pseudouridine synthase B (truB) CSB-YP409016NFA
CSB-EP409016NFA
CSB-BP409016NFA
CSB-MP409016NFA
CSB-EP409016NFA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitratiruptor sp. tRNA (guanine-N (1)-)-methyltransferase CSB-YP409017NFA
CSB-EP409017NFA
CSB-BP409017NFA
CSB-MP409017NFA
CSB-EP409017NFA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitratiruptor sp. Pantothenate synthetase (panC) CSB-YP409018NFA
CSB-EP409018NFA
CSB-BP409018NFA
CSB-MP409018NFA
CSB-EP409018NFA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitratiruptor sp. Molybdenum cofactor biosynthesis protein A (moaA) CSB-YP409019NFA
CSB-EP409019NFA
CSB-BP409019NFA
CSB-MP409019NFA
CSB-EP409019NFA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitratiruptor sp. Biotin synthase (bioB) CSB-YP409020NFA
CSB-EP409020NFA
CSB-BP409020NFA
CSB-MP409020NFA
CSB-EP409020NFA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中，除了表達系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示，可以幫助你做出明智的決策。

第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條：如何選擇合適的表達載體?

表達載體是一種DNA分子，它攜帶特定的基因進入宿主細胞，并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達，經(jīng)過多年的發(fā)展，表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示，本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條：如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用，對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.jhhuazhong.com/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達服務(wù)，您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問，涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等，您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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