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重組蛋白

產品名稱 貨號 規(guī)格
Recombinant Methanococcus maripaludis Replication factor C small subunit (rfcS) CSB-YP407910MNR
CSB-EP407910MNR
CSB-BP407910MNR
CSB-MP407910MNR
CSB-EP407910MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Ribosomal RNA large subunit methyltransferase E (rlmE) CSB-YP407911MNR
CSB-EP407911MNR
CSB-BP407911MNR
CSB-MP407911MNR
CSB-EP407911MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis UPF0285 protein MmarC7_1666 (MmarC7_1666) CSB-YP407912MNR
CSB-EP407912MNR
CSB-BP407912MNR
CSB-MP407912MNR
CSB-EP407912MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Putative HTH-type transcriptional regulatory protein MmarC7_1702 (MmarC7_1702) CSB-YP407913MNR
CSB-EP407913MNR
CSB-BP407913MNR
CSB-MP407913MNR
CSB-EP407913MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes UPF0758 protein Asuc_0013 (Asuc_0013) CSB-YP407914AUG
CSB-EP407914AUG
CSB-BP407914AUG
CSB-MP407914AUG
CSB-EP407914AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes 50S ribosomal protein L10 (rplJ) CSB-YP407915AUG
CSB-EP407915AUG
CSB-BP407915AUG
CSB-MP407915AUG
CSB-EP407915AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Proline--tRNA ligase (proS), partial CSB-YP407916AUG
CSB-EP407916AUG
CSB-BP407916AUG
CSB-MP407916AUG
CSB-EP407916AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Large-conductance mechanosensitive channel (mscL), partial CSB-YP407917AUG1
CSB-EP407917AUG1
CSB-BP407917AUG1
CSB-MP407917AUG1
CSB-EP407917AUG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes ATP synthase epsilon chain (atpC) CSB-YP407918AUG
CSB-EP407918AUG
CSB-BP407918AUG
CSB-MP407918AUG
CSB-EP407918AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP407919AUG
CSB-EP407919AUG
CSB-BP407919AUG
CSB-MP407919AUG
CSB-EP407919AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Protein CyaY (cyaY) CSB-YP407920AUG
CSB-EP407920AUG
CSB-BP407920AUG
CSB-MP407920AUG
CSB-EP407920AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Sulfurtransferase TusA homolog (tusA) CSB-YP407921AUG
CSB-EP407921AUG
CSB-BP407921AUG
CSB-MP407921AUG
CSB-EP407921AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Phosphoglycerate kinase (pgk) CSB-YP407922AUG
CSB-EP407922AUG
CSB-BP407922AUG
CSB-MP407922AUG
CSB-EP407922AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Glutamate--tRNA ligase (gltX) CSB-YP407923AUG
CSB-EP407923AUG
CSB-BP407923AUG
CSB-MP407923AUG
CSB-EP407923AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Na (+)-translocating NADH-quinone reductase subunit A CSB-YP407924AUG
CSB-EP407924AUG
CSB-BP407924AUG
CSB-MP407924AUG
CSB-EP407924AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Ribosomal protein L11 methyltransferase (prmA) CSB-YP407925AUG
CSB-EP407925AUG
CSB-BP407925AUG
CSB-MP407925AUG
CSB-EP407925AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Phosphopantetheine adenylyltransferase (coaD) CSB-YP407926AUG
CSB-EP407926AUG
CSB-BP407926AUG
CSB-MP407926AUG
CSB-EP407926AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes 30S ribosomal protein S18 (rpsR) CSB-YP407927AUG
CSB-EP407927AUG
CSB-BP407927AUG
CSB-MP407927AUG
CSB-EP407927AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes 30S ribosomal protein S2 (rpsB) CSB-YP407928AUG
CSB-EP407928AUG
CSB-BP407928AUG
CSB-MP407928AUG
CSB-EP407928AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Ribonuclease H (rnhA) CSB-YP407929AUG
CSB-EP407929AUG
CSB-BP407929AUG
CSB-MP407929AUG
CSB-EP407929AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產生大量蛋白質、修改基因序列和制造有用的商業(yè)產品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據他們開發(fā)重組蛋白的經驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產,在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.jhhuazhong.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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